Abstract
The aims of this study were (i) to compare PCP removal (100 mg L−1) by two bacterial consortia B1 and B2 in sterile wastewater (STWW) and liquid mineral medium (MSM), (ii) PCP effect in biofilm formation and antimicrobial susceptibility. PCP removal was measured by high-performance liquid chromatography (HPLC) during 168 h at 30 °C. Biofilm formation was assessed with two approaches: Congo Red Agar and Microtiter-plate. Antimicrobial susceptibility was determined by the agar disc diffusion technique. The results showed that the PCP removal for consortium B1 and B2 after 168 h was 70 and 97.5% in STWW; 62.2 and 85.5% in MSM, respectively. In addition, PCP addition showed an increase in biofilm development especially for B2 consortium around 3.5 nm in 100 mg L−1 PCP. PCP added in the Muller Hinton (MH) medium and Gentamicin disc showed a clear increase in diameter of cell lysis around 2 to 4.5 cm.
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Acknowledgements
The authors want to thank the University of Tunis El Manar for granting a PhD studentship to Ms Rim Werheni. Therefore, this research was funded by the Tunisian Ministry of Higher Education and Scientific Research and Water Treatment and Recycling Laboratory, Water Research and Technology Center (CERTE). We thank all the co-workers for their technical support and for their editing of the manuscript.
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Supplementary file 1 (DOCX 474 KB). PCP effects on the biofilm bacteria morphotypes in BHI growth medium at 30 °C for 48 h of incubation.
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Ammeri, R.W., Di Rauso Simeone, G., Hassen, W. et al. Bacterial consortium biotransformation of pentachlorophenol contaminated wastewater. Arch Microbiol 203, 6231–6243 (2021). https://doi.org/10.1007/s00203-021-02589-9
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DOI: https://doi.org/10.1007/s00203-021-02589-9