Abstract
A novel Marinactinospora strain JX35-4T was isolated from red soil which was collected from Wushan, northern Jiangxi Province, China. Analysis of the 16S rRNA gene sequences showed that strain JX35-4T belongs to the genus Marinactinospora and formed a distinct phylogenetic clade with Marinactinospora thermotolerans SCSIO 00652T and Marinactinospora endophytica YIM 690053T with sequence similarity of 96.97% and 96.42%, respectively. The strain was Gram-positive and formed branched substrate hyphae with no fragmentation, and abundant aerial hyphae that differentiated into long spore chains, and short rod-shaped spores. Growth occurred at 20–45 °C, pH 7.0–12.0 and in the presence of 0–7.5% (w/v) NaCl. The genomic DNA G + C content was determined to be 68.3 mol%. The cell wall of strain JX35-4T contained meso-diaminopimelic acid and xylose. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol mannosides and one unidentified phospholipid. The major fatty acids of strain JX35-4T consisted of anteiso-C17:0 and iso-C16:0. Major menaquinones were MK-9(H10), MK-12 and MK-10(H2). Based on the polyphasic data, strain JX35-4T (= CGMCC 4.7382T = DSM 104977T) is concluded to represent a novel species of the genus Marinactinospora, for which the name Marinactinospora rubrisoli sp. nov. is proposed.
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Acknowledgements
The authors are grateful to Prof. Jisheng Ruan (Institute of Microbiology, Chinese Academy of Sciences, IMCAS) for suggesting the Latin name of the species, and to Prof. Chunli Li (IMCAS) for his help in SEM analyses. This work was supported by the National Program on Key Basic Research Project (973 Program, no. 2014CB744400), Major Project of Military Medicine (no. BWS17C030) and the General Financial Grant from the China Postdoctoral Science Foundation (no. 2016M602971).
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Communicated by Erko Stackebrandt.
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Huang, B., Cheng, K., Zhang, L. et al. Marinactinospora rubrisoli sp. nov., isolated from red soil. Arch Microbiol 201, 459–465 (2019). https://doi.org/10.1007/s00203-018-1583-2
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DOI: https://doi.org/10.1007/s00203-018-1583-2