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Microvirga rosea sp. nov.: a nanoparticle producing bacterium isolated from soil of rose garden

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Abstract

A Gram-stain negative, aerobic, non-motile, and rod-shaped novel bacterial strain, designated MAH-2T, was isolated from a soil sample of rose garden and was characterized using a polyphasic approach. The colonies were light pink color, smooth, circular and 0.2–0.6 mm in diameter when grown on nutrient agar for 3 days. Strain MAH-2T grows at 15–40 °C (optimum growth temperature 30 °C), at pH 5.0–7.0 (optimum growth pH 6.5) and at 0–2% NaCl (optimum 0-0.5%). Cell growth occurs on nutrient agar and R2A agar but not on tryptone soya agar, luria–bertani agar and MacConkey agar. The strain was positive for both catalase and oxidase tests. The strain was able to synthesis of silver nanoparticles. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Microvirga and was most closely related to Microvirga soli R491T (96.7% sequence similarity), Microvirga subterranea Fail4T (96.4%), Microvirga guangxiensis 25BT (96.0%) and Microvirga aerophila 5420S-12T (95.9%). The genomic DNA G + C content of isolated strain was determined to be 62.5 mol% and the predominant isoprenoid quinone was Q-10. The major fatty acids were identified as summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c) and C19:0 cyclo ω8c. On the basis of these phenotypic, genotypic, and chemotaxonomic studies and DNA–DNA hybridization results, the isolated strain MAH-2T represents a novel species, for which the name Microvirga rosea sp. nov. is proposed, with MAH-2T as the type strain (= KACC 19290T = CGMCC1.16488T).

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Acknowledgements

This study was performed with the support of the National Research Foundation (NRF) of Korea grant (Project no. NRF-2018R1C1B5041386) funded by Korean government, Republic of Korea.

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Authors and Affiliations

  1. Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong-si, Gyeonggi-do, 17546, Republic of Korea

    Md. Amdadul Huq

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  1. Md. Amdadul Huq
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Correspondence to Md. Amdadul Huq.

Additional information

Communicated by Erko Stackebrandt.

The NCBI GenBank accession number for the 16S rRNA gene sequence of strains MAH-2T is KY964282 and the digital protologue database (DPD) Taxon Number is TA00610.

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Huq, M.A. Microvirga rosea sp. nov.: a nanoparticle producing bacterium isolated from soil of rose garden. Arch Microbiol 200, 1439–1445 (2018). https://doi.org/10.1007/s00203-018-1558-3

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  • DOI: https://doi.org/10.1007/s00203-018-1558-3

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