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Extra-slow-growing Tardiphaga strains isolated from nodules of Vavilovia formosa (Stev.) Fed.

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Abstract

Eleven extra-slow-growing strains were isolated from nodules of the relict legume Vavilovia formosa growing in North Ossetia (Caucasus) and Armenia. All isolates formed a single rrs cluster together with the type strain Tardiphaga robiniae LMG 26467T, while the sequencing of the 16S–23S rDNA intergenic region (ITS) and housekeeping genes glnII, atpD, dnaK, gyrB, recA and rpoB divided them into three groups. North Ossetian isolates (in contrast to the Armenian ones) were clustered separately from the type strain LMG 26467T. However, all isolates were classified as T. robiniae because the DNA–DNA relatedness between them and the type strain LMG 26467T was 69.6 % minimum. Two symbiosis-related genes (nodM and nodT) were amplified in all isolated Tardiphaga strains. It was shown that the nodM gene phylogeny is similar to that of ITS and housekeeping genes. The presence of the other symbiosis-related genes in described Tardiphaga strains, which is recently described genus of rhizobia, as well as their ability to form nodules on any plants are under investigation.

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Acknowledgments

We thank Pia Clercx for performing DNA–DNA hybridizations and excellent technical assistance. This work was partially supported by the Ministry of Education and Sciences of the Russian Federation (Agreement No. 14.604.21.0024, RFMEFI60414X0024) and by the European Union’s Seventh Framework Programme (BRIO project, grant KBBE 2010-4-266106). The nodM and nodT sequencing analysis was supported by the Russian Science Foundation (Grant No. 14-26-00094).

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Authors and Affiliations

  1. All-Russia Research Institute for Agricultural Microbiology (ARRIAM), St. Petersburg, Russian Federation

    Vera I. Safronova, Irina G. Kuznetsova, Anna L. Sazanova, Anastasiia K. Kimeklis, Andrey A. Belimov, Evgeny E. Andronov, Alexander G. Pinaev & Igor A. Tikhonovich

  2. Gorsky State Agrarian University, Vladikavkaz, Russian Federation

    Andrey R. Pukhaev

  3. North-Ossetian Nature Reserve, Alagir, Republic North-Ossetia-Alania, Russian Federation

    Konstantin P. Popov

  4. Institute of Botany of National Academy of Sciences of Republic Armenia, Yerevan, Republic of Armenia

    Janna A. Akopian

  5. Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium

    Anne Willems

  6. Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russian Federation

    Igor A. Tikhonovich

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  1. Vera I. Safronova
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  2. Irina G. Kuznetsova
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  3. Anna L. Sazanova
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  4. Anastasiia K. Kimeklis
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  5. Andrey A. Belimov
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  6. Evgeny E. Andronov
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  7. Alexander G. Pinaev
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  8. Andrey R. Pukhaev
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  9. Konstantin P. Popov
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  10. Janna A. Akopian
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  12. Igor A. Tikhonovich
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Correspondence to Vera I. Safronova.

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Communicated by Erko Stackebrandt.

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Safronova, V.I., Kuznetsova, I.G., Sazanova, A.L. et al. Extra-slow-growing Tardiphaga strains isolated from nodules of Vavilovia formosa (Stev.) Fed.. Arch Microbiol 197, 889–898 (2015). https://doi.org/10.1007/s00203-015-1122-3

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