Abstract
Halophilic archaeal strain YC25T was isolated from Yuncheng salt lake in Shanxi, China. Cells of strain YC25T were observed to be pleomorphic rods, stained Gram-negative, and formed red-pigmented colonies on solid media. Strain YC25T was found to be able to grow at 25–50 °C (optimum 37 °C), at 1.4–4.8 M NaCl (optimum 1.7 M), at 0–1.0 M MgCl2 (optimum 0.01 M), and at pH 5.5–9.0 (optimum pH 6.5). The cells lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 8 % (w/v). The major polar lipids of the strain were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS), sulfated galactosyl mannosyl glucosyl diether (S-TGD-1), sulfated mannosyl glucosyl diether (S-DGD-1), galactosyl mannosyl glucosyl diether (TGD-1), mannosyl glucosyl diether (DGD-1), and an unknown diglycosyl diether (DGD-2) chromatographically identical to those of Halorussus rarus CGMCC 1.10122T. The 16S rRNA gene and rpoB′ gene of strain YC25T were phylogenetically related to the corresponding genes of Halorussus rarus CGMCC 1.10122T (94.3–95.4 and 91.5 % nucleotide identity, respectively). The DNA G+C content of strain YC25T was determined to be 63.3 mol%. The phenotypic, chemotaxonomic, and phylogenetic properties suggested that strain YC25T (=CGMCC 1.12122T = JCM 18363T) represents a new species of Halorussus, for which the name Halorussus ruber sp. nov. is proposed.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31370054), the grant from China Ocean Mineral Resources R&D Association (COMRA) Special Foundation (DY125-15-R-03), and the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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Communicated by Erko Stackebrandt.
Phase-contrast micrograph of strain YC25T, thin-layer chromatograms of the polar lipids extracted from strain YC25T and some related haloarchaea, maximum-parsimony and neighbor-joining phylogenetic tree reconstructions based on 16S rRNA gene and rpoB′ gene sequences showing the relationships between strain YC25T and related members within the family Halobacteriaceae are available as supplementary materials.
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Xu, WD., Zhang, WJ., Han, D. et al. Halorussus ruber sp. nov., isolated from an inland salt lake of China. Arch Microbiol 197, 91–95 (2015). https://doi.org/10.1007/s00203-014-1058-z
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DOI: https://doi.org/10.1007/s00203-014-1058-z