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Rhizobium helanshanense sp. nov., a bacterium that nodulates Sphaerophysa salsula (Pall.) DC. in China

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Abstract

Studying rhizobia in the root nodules of Sphaerophysa salsula (Pall.) DC in the northwest of China, we obtained five strains classified as genus Rhizobium on the basis of their 16S rRNA gene sequences. The sequence similarity of strain CCNWQTX14T with the most related species was 99.0%. Further phylogenetic analysis of housekeeping genes (recA and atpD) suggested the five strains comprised a novel lineage within Rhizobium. The nifH and nodD gene sequences of CCNWQTX14T were phylogenetically closely related with those of Sinorhizobium kummerowiae and R. sphaerophysae, respectively. The five strains isolated from different places were also distinct from related Rhizobium species using ERIC fingerprint profiles. The DNA–DNA hybridization value was 41.8% between CCNWQTX14T and Rhizobium sphaerophysae CCNWGS0238T. Our novel strains were only able to form effective nodules on its original host Sphaerophysa salsula. Our data showed that the five Rhizobium strains formed a unique genomic species, for which a novel species Rhizobium helanshanense sp. nov. is proposed. The type strain is CCNWQTX14T (=ACCC 16237T =HAMBI 3083T).

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Acknowledgments

This research was supported by National Science Foundation of China (31125007, 30970003 and 30900215), the Project of Xinjiang Prodution & Corps key laboratory of Protection and Utilization of Biological Resources in Tarim Basin (BRZD1002). We are grateful for the help from Prof. Christopher Rensing and Dr. Monika Walter in editing the manuscript.

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Authors and Affiliations

  1. College of Life Sciences, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, 712100, Shaanxi, China

    Wei Qin, Zhen Shan Deng, Lin Xu, Na Na Wang & Ge Hong Wei

  2. Xinjiang Prodution and Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Alar, 843300, Xinjiang, China

    Lin Xu

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  1. Wei Qin
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  2. Zhen Shan Deng
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  3. Lin Xu
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  4. Na Na Wang
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  5. Ge Hong Wei
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Correspondence to Ge Hong Wei.

Additional information

Communicated by Erko Stackebrandt.

Electronic supplementary material

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203_2011_766_MOESM1_ESM.ppt

Supplementary Figures Comparison of the recA (Fig. S1) and atpD (Fig. S2) gene sequences. Phylogenetic trees showed the relationships among R. helanshanense sp. nov. and the reference strains. Trees were constructed by neighbor-joining method, and bootstrap confidence levels ≥70% were indicated at the nodes. Bar, 5% nucleotide substitutions. Supplementary material 1 (PPT 102 kb)

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Qin, W., Deng, Z.S., Xu, L. et al. Rhizobium helanshanense sp. nov., a bacterium that nodulates Sphaerophysa salsula (Pall.) DC. in China. Arch Microbiol 194, 371–378 (2012). https://doi.org/10.1007/s00203-011-0766-x

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