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Adhesion properties of Lactobacillus casei strains to resected intestinal fragments and components of the extracellular matrix

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Abstract

Adhesion to intestinal epithelium is an outcome property for the selection of probiotic lactic acid bacteria strains. We have analyzed the adhesion properties of a collection of Lactobacillus casei strains from different origins, ranging from cheese isolates to commercial probiotics. Analysis of the surface characteristics of the strains by measuring adhesion to solvents (MATS test) showed that most of the strains have a basic and hydrophobic surface. The strains were able to bind ex vivo to human colon fragments at different levels and, in most cases, this adhesion correlated with the ability to in vitro binding of mucin. Attachment to this later substrate was not enhanced by growing the cells in the presence of mucin and was independent of proteinaceous factors. On the contrary, adhesion to other extracellular matrix components, such as collagen, fibronectin, or fibrinogen was partially or totally dependent on the presence of surface proteins. These results show that most of L. casei strains have in their surfaces factors that promote binding to intestinal epithelium, however, no clear correlation appears to exist between the origin of the strains and their adhesion capacities.

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Abbreviations

LAB:

Lactic acid bacteria

MATS:

Microbial adhesion to solvents test

ECM:

Extracellular matrix

PBS:

Phosphate-buffered saline

LDH:

Lactate dehydrogenase

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Acknowledgments

This work was financed by funds of the Spanish Ministerio de Ciencia e Innovación (AGL2004-00176 and Consolider Fun-c-Food CSD2007-00063). Diego Muñoz-Provencio was supported by a pre-doctoral fellowship from the Conselleria de Cultura of the Generalitat Valenciana. We thank Siv Ahrne and Goran Molin (Department of Food Technology, Lund University, Lund, Sweden) for providing us with the strains L. plantarum 299v and 299v Adh−.

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Authors and Affiliations

  1. Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos-CSIC, P.O. Box 73, Burjassot, 46100, Valencia, Spain

    Diego Muñoz-Provencio, Gaspar Pérez-Martínez & Vicente Monedero

  2. Digestive System Research Unit, University Hospital Vall d’Hebron, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (Ciberehd), Barcelona, Spain

    Marta Llopis, María Antolín & Francisco Guarner

  3. Department of Pathological Anatomy, University Hospital Vall d’Hebron, Paseo Vall d’Hebron 129, 08035, Barcelona, Spain

    Inés de Torres

Authors
  1. Diego Muñoz-Provencio
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  2. Marta Llopis
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  3. María Antolín
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  4. Inés de Torres
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  5. Francisco Guarner
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  6. Gaspar Pérez-Martínez
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  7. Vicente Monedero
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Correspondence to Vicente Monedero.

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Communicated by Erko Stackebrandt.

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Muñoz-Provencio, D., Llopis, M., Antolín, M. et al. Adhesion properties of Lactobacillus casei strains to resected intestinal fragments and components of the extracellular matrix. Arch Microbiol 191, 153–161 (2009). https://doi.org/10.1007/s00203-008-0436-9

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  • DOI: https://doi.org/10.1007/s00203-008-0436-9

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