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Gene structure and transcriptional regulation specific to the groESL operon from the psychrophilic bacterium Colwellia maris

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Abstract

The groESL operon of a psychrophilic bacterium, Colwellia maris, was cloned and sequenced. The operon contains two ORFs of 291 bp and 1,650 bp separated by 210 bp. Northern blot analysis suggested that the groESL operon was transcribed as a bicistronic mRNA, and that the amount of mRNA markedly increased after the temperature was raised from 10 °C to 20 °C. Although the optimum temperatures for GroESL function are different in psychrophilic, mesophilic, and thermophilic bacteria, the deduced amino acid sequences of C. maris GroES and GroEL showed remarkably high similarity with those of GroES and GroEL from mesophilic and thermophilic bacteria. A putative promoter similar to the Escherichia coli σ32 consensus sequence was identified. One specific feature of C. maris groESL was that in the putative untranslated region the G+C content was about 24 mol%, which is much lower than that of mesophilic bacteria such as E. coli. The low G+C content may be important for maintaining transcription at low temperatures.

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Acknowledgements

We thank Yoshitaka Nishiyama for critical discussions and help in the present studies.

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  1. Eugene Hayato Morita

    Present address: Center for Gene Research, Ehime University, 790-8566 , Matsuyama, Ehime, Japan

Authors and Affiliations

  1. Graduate School of Science and Engineering, Ehime University, 790-8577 , Matsuyama, Ehime, Japan

    Seiji Yamauchi

  2. Laboratory of Environmental Molecular Biology, Hokkaido University, Graduate School of Environmental Earth Science, 060-0810 , Sapporo, Hokkaido, Japan

    Hidetoshi Okuyama

  3. Department of Chemistry, Ehime University, Faculty of Science, 790-8577 , Matsuyama, Ehime, Japan

    Eugene Hayato Morita & Hidenori Hayashi

  4. Satellite Venture Business Laboratory, Ehime University, 790-8577 , Matsuyama, Ehime, Japan

    Eugene Hayato Morita & Hidenori Hayashi

  5. Department of Chemistry and Satellite Venture Business Laboratory, Ehime University, 790-8577 , Matsuyama, Ehime, Japan

    Hidenori Hayashi

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  1. Seiji Yamauchi
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Correspondence to Hidenori Hayashi.

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Yamauchi, S., Okuyama, H., Morita, E.H. et al. Gene structure and transcriptional regulation specific to the groESL operon from the psychrophilic bacterium Colwellia maris . Arch Microbiol 180, 272–278 (2003). https://doi.org/10.1007/s00203-003-0587-7

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  • DOI: https://doi.org/10.1007/s00203-003-0587-7

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