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Exercise-induced inhibition of remodelling is focally offset with fatigue fracture in racehorses

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Abstract

Summary

Bone remodelling is inhibited by high repetitive loading. However, in subchondral bone of racehorses in training, eroded surface doubled in association with fatigue fracture and there was greater surrounding trabecular bone volume suggesting trabecular modelling unloads the bone focally, allowing damage repair by remodelling.

Introduction

Remodelling replaces damaged bone with new bone but is suppressed during high magnitude repetitive loading when damage is most likely. However, in cortical bone of racehorses, at sites of fatigue fracture, focal porosity, consistent with remodelling, is observed in proportion to the extent of surrounding callus. Focal areas of porosity are also observed at sites of fatigue damage in subchondral bone. We hypothesised that fatigued subchondral bone, like damaged cortical bone, is remodelled focally in proportion to the modelling of surrounding trabecular bone.

Methods

Eroded and mineralizing surfaces and bone area were measured using backscattered scanning electron microscopy of post-mortem specimens of the distal third metacarpal bone in 11 racehorses with condylar fractures (cases) and eight racehorses in training without fractures (controls).

Results

Cases had a two-fold greater eroded surface per unit area at the fracture site than controls (0.81 ± 0.10 vs. 0.40 ± 0.12 mm−1, P = 0.021) but not at an adjacent site (0.22 ± 0.09 vs. 0.30 ± 0.11 mm−1, P = 0.59). Area fraction of surrounding trabecular bone was higher in cases than controls (81 ± 2 vs. 72 ± 2 %, P = 0.0020) and the eroded surface at the fracture site correlated with the surrounding trabecular area (adjusted R 2 = 0.63, P = 0.0010).

Conclusion

In conclusion, exercise-induced inhibition of remodelling is offset at sites of fatigue fracture. Modelling of trabecular bone may contribute to unloading these regions, allowing repair by remodelling.

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Acknowledgments

Funding was provided by the Rural Industries Research and Development Corporation of the Australian Government and Racing Victoria Limited. We thank Mark Forwood for advice on the manuscript.

Conflicts of interest

None.

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Authors and Affiliations

  1. Faculty of Veterinary Science, Equine Centre, University of Melbourne, 250 Princes Hwy, Werribee, Victoria, 3030, Australia

    R. C. Whitton & G. A. Anderson

  2. Faculty of Veterinary Science, University of Melbourne, Park Drive and Flemington Rd, Parkville, Victoria, 3010, Australia

    M. Mirams & E. J. Mackie

  3. Department of Endocrinology and Medicine, Austin Health, University of Melbourne, 300 Waterdale Rd, Heidelberg West, Victoria, 3081, Australia

    E. Seeman

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  1. R. C. Whitton
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  2. M. Mirams
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  3. E. J. Mackie
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  5. E. Seeman
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Correspondence to R. C. Whitton.

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Whitton, R.C., Mirams, M., Mackie, E.J. et al. Exercise-induced inhibition of remodelling is focally offset with fatigue fracture in racehorses. Osteoporos Int 24, 2043–2048 (2013). https://doi.org/10.1007/s00198-013-2291-z

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  • DOI: https://doi.org/10.1007/s00198-013-2291-z

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