In vivo performance of a novel silk fibroin scaffold for partial meniscal replacement in a sheep model

Purpose Due to the negative effects of meniscectomy, there is a need for an adequate material to replace damaged meniscal tissue. To date, no material tested has been able to replace the meniscus sufficiently. Therefore, a new silk fibroin scaffold was investigated in an in vivo sheep model. Methods Partial meniscectomy was carried out to the medial meniscus of 28 sheep, and a scaffold was implanted in 19 menisci (3-month scaffold group, n = 9; 6-month scaffold group, n = 10). In 9 sheep, the defect remained empty (partial meniscectomy group). Sham operation was performed in 9 animals. Results The silk scaffold was able to withstand the loads experienced during the implantation period. It caused no inflammatory reaction in the joint 6 months postoperatively, and there were no significant differences in cartilage degeneration between the scaffold and sham groups. The compressive properties of the scaffold approached those of meniscal tissue. However, the scaffolds were not always stably fixed in the defect, leading to gapping between implant and host tissue or to total loss of the implant in 3 of 9 cases in each scaffold group. Hence, the fixation technique needs to be improved to achieve a better integration into the host tissue, and the long-term performance of the scaffolds should be further investigated. Conclusion These first in vivo results on a new silk fibroin scaffold provide the basis for further meniscal implant development. Whilst more data are required, there is preliminary evidence of chondroprotective properties, and the compressive properties and biocompatibility are promising.


Introduction
The menisci play a key role in load distribution in the knee joint [20,36]. Meniscal lesions treated by partial or total meniscectomy compromise this function. It is well known that this can hasten the onset of osteoarthritis [3], which makes the need for new treatment strategies of great clinical relevance. Therefore, various materials have been developed and investigated to fill meniscal defects [33]. However, only few strategies showed a chondroprotective function [11,34]. To date, scaffolds made of processed natural materials, e.g. small intestine submucosa, tendon tissue and isolated tissue components have also been used as meniscus replacement [26]. The Collagen Meniscus Implant (CMI, also known as Menaflex Ò ) has already been used clinically for some years. Although some good results have been reported concerning tissue regeneration and pain relief scoring in several clinical trials [16,31,32,42] and experimentally [14], the mechanical functionality and chondroprotective effect of the implant has not yet been confirmed experimentally or in clinical studies [25,28]. The implantation of the CMI resulted in the formation of scar tissue, which has inferior mechanical properties compared to meniscal tissue [28]. Therefore, it is assumed that the CMI contributes little to load transmission in the knee joint [4]. In addition, non-degradable and biodegradable synthetic polymers have been developed for the replacement of damaged meniscal tissue. Amongst this scaffold class, a polycaprolactone-polyurethane scaffold (Actifit Ò ) has already been investigated in several preclinical and clinical studies [13,24,40]. The mechanical properties of the polycaprolactone-polyurethane material are significantly less than those of the meniscus [38], and whether this scaffold can protect the articular cartilage from degeneration, and thus has advantages over partial meniscectomy, still needs to be confirmed [24].
Because to date no material has clearly demonstrated satisfactory replacement of damaged meniscal tissue, the present study investigated a novel scaffold formed from the structural protein, silk fibroin. Several materials manufactured from silk fibroin have already demonstrated good biocompatibility and excellent mechanical properties in other biomedical applications [1,23]. The aim of this in vivo study was to analyse the suitability of the new silk fibroin scaffolds in partial meniscus replacement. Specifically, we hypothesized that implantation of a silk fibroin scaffold as a partial meniscal replacement device prevents the development of articular cartilage degeneration by comparison with partial meniscectomy.

Study design
Thirty-seven skeletally mature (4.2 ± 0.9 years) female Merino sheep with an average weight of 88 ± 11 kg were used for the investigations. The animals were randomly divided into four groups (Table 1). All groups underwent a medial arthrotomy including the release of the medial collateral ligament. Group 1 (3-month scaffold group; sc3), group 3 (6-month partial meniscectomy group; pm) and group 4 (6-month scaffold group; sc6) underwent partial meniscectomy, and in groups 1 and 4, a silk scaffold was then implanted into the meniscal defect. Group 2 (6-month sham surgery group; sh) served as a control to demonstrate that the arthrotomy itself does not lead to degenerative changes in the stifle joint. After 3 and 6 months, respectively, the animals were killed and both hind limbs were collected and prepared for macroscopic, biomechanical, immunochemical and histological analysis.

Materials
Silk fibroin scaffolds were manufactured from Bombyx mori raw silk. After degumming of raw silk fibres as described by Hakimi et al. [12], the resulting silk fibroin was processed into a porous matrix. Silk fibroin devices were then cut to size (Fig. 1a) and immersed in 0.9 % phosphate-buffered saline before being irradiated at 25 KGy to sterilize. Sterilized scaffolds were stored at 4°C until used for surgery. An original sheep meniscus taken from the same race and age as used for the animal study Group 2 (sh) Sham 6 months 9 9 Group 3 (pm) Partial meniscectomy 6 months 9 8 Group 4 (sc6) Scaffold 6 months 10 9 * n corr corrected number after exclusion of two animals was employed as a template for the scaffold mould. Since shape and size of the menisci varied only little between animals of the same age and race, this method provided the best possible fit for the animal experiment. Prior to the in vivo study the scaffolds' structural, mechanical and biological properties were investigated. The porosity of the silk fibroin scaffolds was determined using high-resolution micro-CT (SkyScan, Belgium). The scaffolds showed a pore size ranging from 150 to 300 lm, a porosity of 85 % and very smooth surfaces (Fig. 1a, b). Cylindrical samples of the scaffold were tested in a materials testing machine (Z010, Zwick GmbH & Co. KG, Germany) and the compression test (unconfined compression) revealed a Young's modulus of 1.1 ± 0.2 MPa at strains \10 %, which was in the range of moduli obtained for human menisci at 12 % strain [6].

Operation
All sheep were operated on the right stifle joint under general anaesthesia. After medial incision of skin and fasciae, the medial collateral ligament was released including its femoral bony attachment to gain access to the medial compartment of the joint. Then, the joint capsule was opened. Flexion and external rotation of the stifle joint enabled access to the medial meniscus. In the meniscectomy group, and both scaffold groups, a 9 mm 9 7 mm 9 5 mm piece of meniscus was resected at the anterior horn of the medial meniscus (Fig. 1c). A small millimetre scale was used to achieve these dimensions as close as possible. The meniscal explant served as a template to adjust the shape of the scaffold implant by use of a scalpel. The adjusted scaffold was implanted into the defect of the scaffold group animals with two non-penetrating horizontal mattress sutures. The meniscal defect remained untreated in the meniscectomy group. In the sham group, no intervention at the meniscus was carried out. The degree of cartilage degeneration in the visible area of the joint was recorded for each sheep. After closure of the joint capsule, the medial collateral ligament was reattached with a claw plate and a bicortical bone screw. Finally, fasciae and skin were sutured in separate layers. Full weight bearing was permitted immediately after surgery, and there was no restriction to the movement of the operated limb.

Macroscopic evaluation
Both the knee region and the stifle joint itself were examined macroscopically after killing. In the area of the surgical access, particular attention was paid to any disturbance to wound healing and signs of inflammation. Changes to the joint capsule such as colour changes, thickening, fibrosis, calcification or villus formation were recorded. The consistency of the synovial fluid was also evaluated. The scaffold's shape and its attachment to the meniscal tissue were assessed and observations were noted. The articular cartilage of the tibial plateau and femoral condyles was examined for signs of degeneration.

Biomechanical testing
Indentation testing of the tibial condyle was performed to determine the equilibrium modulus (E eq ) of the articular cartilage in groups 2 (sh), 3 (pm) and 4 (sc6). Medial tibial condyles were separated from the tibia and mounted in a transparent testing chamber filled with saline. The testing chamber allowed exact alignment of the cartilage surface parallel to the indenter at all locations. For each tibial plateau, three locations were tested (Fig. 3a). The indentation tests were performed using a materials testing machine (Z010, Zwick GmbH & Co. KG, Germany) with a flat-ended steel indenter ([ 1.5 mm). The compressive force was measured by a 50-N load cell KAP-Z, AST GmbH, Germany, 0.05 % accuracy) and the displacement by a laser sensor (optoNCDT2200-20, Micro-Epsilon GmbH &Co.KG, Germany, 0.3 lm resolution, 0.03 % accuracy). Cartilage thickness was measured according to the needle method described by Hoch et al. [17]. After applying a 0.2-N preload, the cartilage was loaded with a constant speed (100 % of cartilage thickness per minute) until a load of 4.8 N was applied. The resulting strain was sustained for 20 min, and the decrease in force was recorded. The equilibrium modulus (E eq ) of the cartilage was calculated using the equation according to Hooke's law: where F R = equilibrium force, d = cartilage thickness, A = surface area of the indenter, Dl = indentation depth. Tissue punches ([ 3 mm) of meniscus and scaffold were mechanically tested under confined-compression in the materials testing machine to determine the equilibrium modulus (E eq ). The punches were removed from the resected meniscus tissue after surgery and cut coplanar to the tibial surface. The resulting cylinders were placed into a 3-mm diameter hole in a plastic test chamber with the cut surface facing down. The test chamber was filled with saline. A porous ceramic cylinder was placed between specimen and stamp to allow fluid flow during testing. After applying a 0.5-N preload, a stress-relaxation test under confined-compression was performed at 20 % strain. The strain was kept constant for 15 min, and the decrease in force was recorded over the testing period. Similarly, samples of the scaffolds before and after implantation were tested with the smooth surface facing down during testing to allow fluid flow out of the scaffolds. The equilibrium modulus of the samples was calculated for each strain level as described above.
Macroscopic grading of articular cartilage after staining with India Ink India Ink (Pelikan mbH & Co. KG., Germany) was applied to the cartilage surfaces of formaldehyde-fixed femoral condyles and tibial plateaus to facilitate the macroscopic grading of cartilage degeneration. The carbon particles of the ink bound to degenerated and fibrillated cartilage areas but not to the smooth, intact cartilage surface. One drop of ink was applied to each specimen and gently rubbed in using a wet cloth. After brief rinsing under running water, photographs of condyles and plateaus were taken. Using an image analysis software (Leica GmbH, Germany), the area of degenerated (i.e. ink stained) cartilage was determined. Total cartilage area was also determined. Then, the proportion of degenerated cartilage area in total cartilage area was calculated and served as a measure for the degree of cartilage degeneration.

Histology
Synovial membranes, scaffolds and tibial cartilage were assessed histologically. Some problems during sample processing, e.g. breaking of the cartilage-subchondralbone slices or separation of the cartilage layer occurred. Therefore, the number of slices that were evaluated per group was low in some cases. Staining with haematoxylin eosin (H&E) was performed for differential analysis of tissue structures, and cartilage sections were additionally stained with Safranin-O fast green (Saf-O) for the assessment of glycosaminoglycan content. Sections of synovial membrane, scaffold and surrounding meniscal tissue were analysed qualitatively with a light microscope (Leica GmbH, Germany). The tibial cartilage sections of groups 2 (sh), 3 (pm) and 4 (sc6) were assessed semiquantitatively using the Mankin grading system, and the Fibrillation Index (FI, quotient of cartilage surface and tidemark lengths) of the cartilage was determined histomorphometrically [27,30].

Institutional review board
All procedures were performed according to the national and international regulations for the care and use of laboratory animals and were approved by the Regional Administrative Authority in Tübingen, Germany (registration number 1020).

Statistical analysis
A power analysis was designed based on the results of an earlier study on meniscal replacement in an ovine model [24], which resulted in a required group size of seven animals. To allow for the possibility of loss of animals during the study period, we increased the group size to at least nine animals. Statistical analysis was performed using SPSS Statistics (IBM SPSS Statistics, USA). Since the data were not normally distributed, a Mann-Whitney U test was performed to determine differences between the groups. To detect differences within each group a Wilcoxon signedrank test was performed. In all cases, a significance level of p = 0.05 was used.

Clinical observations
All animals were partially weight bearing after 4 days and fully weight bearing without visible lameness by 7 days postoperatively. No disturbance of wound healing occurred, and all animals showed a good general condition for the whole test period. One animal died during anaesthesia due to apnoea and cardiac arrest. The physical condition of two animals deteriorated 3 weeks postoperatively. These animals had to be euthanized prior to the termination of the study. In both cases, no obvious correlation between the physical deterioration and the surgical procedure was found. Thus, the number of evaluated animals was reduced to n corr = 34 (Table 1).

Macroscopic findings
In post-mortem, no signs of inflammation were visible in the knee region in any of the four groups. The joint capsule showed a moderate, fibrotic thickening at the medial aspect of the joint in most cases, but no other changes were visible macroscopically. The synovial fluid of all animals appeared normal, being of clear, light yellowish to light reddish colour and of a characteristic viscous consistency. The scaffold was in place in six animals of the 3-month, and in six animals of the 6-month, scaffold groups (Fig. 2e, f). In some of these animals, the scaffold filled the defect completely, but in some animals, the scaffold was not firmly attached to the meniscus, particularly at the posterior edge. In both scaffold groups (groups 1 and 4), the scaffold did not fill the meniscal defect in three animals at termination of the study. Since it is not known at which point the scaffolds had dislocated from the defect, these six animals were excluded from further analysis. No regenerative tissue was found in the meniscal defects of the partial meniscectomy group. Large inter-individual differences in the macroscopic degree of cartilage degeneration were visible, consistent with the observations recorded during the surgical procedure. Between the sham and the 6-month scaffold groups, no differences in the degree of cartilage degeneration were observed macroscopically (Fig. 2a-d).

Indentation test of medial tibial condyle
At the measuring point underneath the meniscal defect (MP 1), the median E eq of the partial meniscectomy group was significantly lower than the modulus of the sham group (p \ 0.05, Fig. 3). After 6 months, the median modulus of the cartilage in the group treated with a scaffold was not statistically different from that of the sham group. The median E eq of the sham group was significantly lower than

Stress-relaxation test of meniscus and scaffold
Due to difficulties in preparation of some scaffold samples, only four of the three and four of the 6-month implanted scaffolds were included in the evaluation of the stressrelaxation test. As expected, the meniscal tissue showed a significantly higher equilibrium modulus than the scaffold at all strain levels (p \ 0.05, Fig. 4). However, the equilibrium modulus of the scaffolds implanted in the stifle joint increased over the implantation period, and after 6 months of implantation, there was no longer a significant difference between the meniscal tissue (95 kPa) and the scaffold (76 kPa).

Macroscopic grading of articular cartilage after staining with India Ink
The degenerated cartilage area on the medial femoral condyles of the partial meniscectomy group was almost twice as large as the degenerated cartilage area of the sham and the 6-month scaffold group (p \ 0.05, Fig. 5e). Single animals showed particularly extensive cartilage degeneration (Fig. 5a). The degenerated cartilage area of the medial femoral condyles was thus significantly larger in the partial meniscectomy group than in the sham group. The medial tibial condyles of the partial meniscectomy group also showed considerably more degenerative changes than the condyles of the sham and the 6-month scaffold group (Fig. 5f), although these differences were not statistically significant. There were no statistical differences between all groups in the lateral compartment (Fig. 5e, f).
Biochemical findings IL 1b concentrations in the synovial fluid were below the detection limit of the ELISA kit pre-and postoperatively in all groups. The IL 6 concentrations were in a relatively narrow range (8.4-11.4 pg/ml) both pre-and postoperatively with no statistical difference between the groups. The PGE 2 concentrations of the individual animals varied widely pre-and postoperatively in all four groups (0-625 pg/ml). An increase in the PGE 2 content occurred postoperatively in the 3-month scaffold group to 400 % of the intraoperative level. Statistical significance could not be tested in this case due to a low number of samples (n = 4), which was caused by insufficient retrieval of synovial fluid. However, the level dropped to intraoperative values after 6 months.

Histological findings
A hyperplasia of the synovial cells and a villous proliferation of the synovial membrane were visible in individual animals of all four groups but was most frequently observed in the 3-month scaffold group. In this group, occasional inflammatory cells were found in the synovial membrane of three animals. No inflammatory cells occurred in the sham, partial meniscectomy and 6-month scaffold group.
In both scaffold groups, no signs of inflammation were present in the meniscal tissue that had been in contact with the scaffold in vivo. The nature of the single scaffolds differed greatly in both scaffold groups ( Table 2). The surfaces of some scaffolds were more or less smooth (Fig. 6a), but other scaffolds had up to 1-mm deep folds at their tibial and femoral surfaces (Fig. 6d, f). Furthermore, differences in pore size and condition of pore walls occurred within the individual scaffolds as well as between different scaffolds. Macropores were formed by pore walls [10 lm. These macropores were compressed to a differing degree, some were empty, but some were filled with amorphous material possibly representing protein-rich fluid, fibroblast-or fibrochondrocyte-like cells and fibrous tissue (Fig. 6a, b, d, e-g). Only single inflammatory cells occurred in the 3-and 6-month implanted scaffolds. Some areas of the scaffolds consisted mainly of micropores ([ \ 10 lm) with very thin pore walls (Fig. 6d, e, g). Most of these micropores were highly compressed and contained neither cells nor tissue (Fig. 6e). Between the scaffolds and the meniscal tissue, a 80-320-lm wide gap was visible in the histological sections and no signs of a fibrous adhesion between scaffold and meniscus could be found histologically (Fig. 6a, b, d, e, g). In general, the condition of the 3-month implanted scaffolds did not differ from the condition of the 6-month implanted scaffolds (Table 2). It was noticeable that the degeneration in the central area was more severe than at the periphery (Figs. 7, 8). Degenerated areas with cartilage fibrillation occurred exclusively in the central part of the condyles and were accompanied by a marked reduction in Saf-O staining (Fig. 7a, b). Cells tended to form clusters in these areas or hypocellularity was observed (Fig. 8c). Peripherally, the interruption of the tidemark by blood vessels was the most frequent change, and often no other changes were visible there (Fig. 8b). Centrally, the median Fibrillation Index and Mankin score of the 6-month scaffold group was much lower than in the sham and partial meniscectomy groups, although the difference was not statistically significant (Fig. 9b, c). Peripherally, the differences in the Fibrillation Index and the Mankin grading between the three groups were much lower (Fig. 9b, c).  (a, b) and tibial condyles (c, d) stained with India Ink; staining was more extensive in the partial meniscectomy group (a, c) than in the 6-month scaffold group (b, d); the relative proportion of degenerative area to total cartilage area is shown in graph e for the femoral condyles and in graph f for the tibial plateaus of sham (sh), partial meniscectomy (pm) and 6-month scaffold (sc6) groups; single values and medians, *p \ 0.05 Table 2 Findings of the histological examination of the scaffolds after 3 and 6 months of implantation

Folds Macropores Micropores Amorphous material
Cells/ tissue

Discussion
The present study demonstrated that after 6-month implantation, the silk fibroin scaffolds showed compressive properties comparable to those of native meniscus. They did not cause an inflammatory reaction in the knee joint, and there was some evidence that they delay the onset of degeneration of the articular cartilage in the operated joint in the medium term partially confirming the hypothesis. The study further showed that the scaffold fixation was not yet satisfactory leading to partial gapping between scaffold and meniscal tissue, or total loss of the scaffold in a minority of cases.
In the present in vivo study, no macroscopic signs of inflammation were present after 3 and 6 months of silk fibroin scaffold implantation. The histological investigations showed only mild alterations in the synovial membrane in both scaffold groups and control groups, and only in the 3-month scaffold group were occasional inflammatory cells found. The findings of the present study are in accordance with numerous in vitro and in vivo studies that have already demonstrated the biocompatibility of silk fibroin materials [2,29,35], and it is notable that other meniscal scaffolds have caused mild to moderate inflammatory reactions in vivo [24,38].  6 Histological sections of scaffolds after three (a-c) and six (d-g) months of implantation stained with HE; a horizontal section of a scaffold of the 3-month scaffold group, b, c are magnifications of a, s suture remnants; d horizontal section of a scaffold of the 6-month scaffold group, e magnification of d, grey arrows folds in the surface of the scaffold, dashed box suturing channel; f vertical section of a scaffold of the 6-month scaffold group, g magnification of f; black arrows pores filled with amorphous and/or fibrous tissue; arrowheads smooth surface of scaffold in contact with meniscus; * artefact When meniscal tissue is damaged, shear forces present a particular challenge to the fixation technique since these forces cause gapping of meniscal tears [9]. Accordingly, a rigid fixation of the scaffold material to the meniscus is very important. In the present study, a non-penetrating technique was chosen to suture the scaffold to the remaining meniscus. With this technique, the suture material did not interact with the articular cartilage and the influence of scaffold implantation on cartilage health could be better determined. On the other hand, the suture material was anchored only in the porous part of the scaffold and not in the more robust scaffold surface. This and immediate postoperative load bearing may have contributed to the dislocation of three scaffolds in each group and to the fact that in some animals, the scaffold was not firmly attached to the meniscal tissue after 6 months of implantation. Additionally, due to the small size of the scaffold, only two sutures were used for fixation. With an increasing number of fixation points, the loads acting on a single suture should decrease and fixation should be more stable. Similar experimental studies with other scaffold materials used three to four sutures to fix the scaffold to the meniscal tissue [24,28]. Nevertheless, insufficient fixation of scaffolds was observed in some cases in these studies too.
Alterations to the fixation technique in combination with changes in scaffold structure such as the integration of a fibre mesh could improve fixation stability of the silk fibroin scaffolds in the future.
The resection of meniscal tissue may not have been performed into the vascularized zone of the meniscus since no regenerative tissue formation was observed in the defect of the partial meniscectomy group. In contrast, varying amounts of regenerative tissue were described in the partial meniscectomy control groups of comparable in vivo studies [7,28], and Maher et al. [24] observed even a considerable amount of regenerative tissue in the partial meniscectomy group, indicating that meniscal resection has been carried out into the vascularized zone. The absence of regenerative tissue in the present study indicates that scaffolds were implanted into the non-vascular part of the meniscus. This, together with the advanced age of the sheep used in this study and hence lower regenerative potential, is likely to have complicated scaffold integration.
Everyday activities like stair climbing cause loads as high as 3.5-fold body weight in the knee joint [21]. Therefore, an adequate initial stability for meniscus replacement materials is essential. Due to the material properties of the scaffolds used in the present study, a very slow degradation rate can be expected [1]. Silk fibroin is known to have very great strength and elasticity [1,23]. Hence, it can be assumed that the silk fibroin scaffold can withstand the high mechanical loads in the longer term. In order to verify whether the scaffolds are suitable to mechanically replace meniscal tissue, the equilibrium moduli of meniscus and scaffold were compared in the present study. It could be shown that the equilibrium modulus of the scaffolds increased over implantation time and approached the modulus of the meniscus. Whether the increase in stiffness is based on an alteration of material properties or on a compaction of the material due to constant loading needs to be further investigated. The mechanical properties of other scaffold materials pre-and postimplantation have only rarely been studied [25]. Tienen et al. [38] described an improvement in the mechanical properties of polycaprolactone-polyurethane and Estane scaffolds over implantation time in a canine model. However, unlike the present study, there were still major differences between the scaffold material and meniscus at the end of the study. No mechanical investigations of a widely used collagen scaffold (CMI) have been published so far [25]. This scaffold has been described as mechanically unstable [28], but nevertheless, it has already been used clinically [4]. Similarly, a polycaprolactone-polyurethane scaffold (ActiFit Ò ) has been used in clinical studies, but no data exist, which present the mechanical properties of the material [39,40]. In some experimental studies with various scaffold materials, even structural changes such as tearing or shrinkage were reported [18,19,24]. An in vivo study with a polycaprolactone-based polyurethane implant for total meniscus replacement showed that all implants were severely damaged after 6 months of implantation [13]. Efe et al. [10] reported shape irregularities and extrusions of the Actifit Ò polyurethane scaffold in a clinical study. Such findings have not been observed in the present study.
The main objective of meniscus replacement is to prevent degeneration of the articular cartilage, which is frequently a consequence of partial meniscectomy in the long term [8]. Therefore, it is necessary to ensure that the replacement material does not cause cartilage defects itself but helps to delay cartilage degeneration. The ovine model is particularly suited to investigate the chondroprotective properties of a material since sheep quickly develop osteoarthritic changes after total or partial meniscectomy [5,24]. In the present study, a highly variable degree of cartilage degeneration was noted in animals at the beginning of the study, which complicated the interpretation of the results. Sheep naturally develop osteoarthritis with increasing age [43], and the mean age of the animals in the study (4.2 years) was quite advanced. This is a major limitation of this study and should be considered in future studies using younger animals, which would provide healthy articular cartilage at the beginning of the study. Nevertheless, on average the comparison of the sham and the 6-month scaffold group showed that the silk fibroin scaffold does not cause articular cartilage degeneration. Additionally, biomechanical investigations, macroscopic grading after India Ink staining and histological analysis of the articular cartilage all indicate that the degenerative changes observed after partial meniscectomy could be delayed by silk fibroin scaffold implantation. The chondroprotective properties should be confirmed over a longer period of time in younger animals. Although other scaffolds have been intensively investigated in experimental studies and some have already been used clinically [15,33,41], the chondroprotective potential of these scaffold Fig. 9 Histological evaluation of the tibial cartilage. a Diagram for histological sampling of the medial tibial condyle with indication of meniscus and scaffold; b Fibrillation Index of the tibial cartilage in the sham (sh), partial meniscectomy (pm) and 6-month scaffold (sc6) group; c Mankin grade (min: 0, max: 14) of the medial tibial cartilage in these groups; single values and median materials has not been unambiguously confirmed so far [10,31,37]. Zaffagnini et al. [42] reported a worsened degeneration score 2 years after lateral CMI implantation in some cases whilst Linke et al. [22] reported no significant difference in Lysholm and IKDC scores between patients receiving the CMI and the meniscectomy control group at 2-years follow up.

Conclusions
The hypothesis that implantation of a silk fibroin scaffold as a partial meniscal replacement device prevents the development of articular cartilage degeneration was partly confirmed. Although there were some indications of chondroprotective instability in scaffold fixation partly compromised the results obtained. Provided the shortcomings of the new material and its implantation technique can be overcome, the mechanical strength, biocompatibility and indications of chondroprotection suggest silk fibroin scaffolds deserve further consideration as an implant for partial meniscal replacement.