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Exposure to Pesticides at Sublethal Level and Their Distribution Within a Honey Bee (Apis mellifera) Colony

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Abstract

Honey bee colonies were exposed to pesticides used in agriculture or within bee hives by beekeepers: coumaphos; diazinon; amitraz or fluvalinate. Samples of bee workers, larvae and royal jelly were analysed using Gas Chromatography-Electron Capture Detection (GC-ECD). Amitraz was quantified using High Performance Liquid Chromatography (HPLC), and Gas Chromatography-Tandem Mass Spectrometry (GC/MS/MS) was used for quantification of diazinon. Sixth day after treatment, coumaphos was found in the royal jelly (250 ng/g) secreted by nurse workers and fluvalinate was found in both bee heads (105 ng/g, 8 days after treatment) and in larvae (110 ng/g, 4 days after treatment). Amitraz residues in all sampled material were below the level of detection of 10 ng/g. Diazinon was not detected in any of the analysed samples. The large quantities of fluvalinate found in bee heads and larvae, the coumaphos residues in royal jelly, and additional potential sub-lethal effects on individual honey bees or brood are discussed.

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Acknowledgments

We would like to acknowledge the assistance provided by Marjan Kokalj for helping with treatments and Vesna Lokar for collecting the samples. This research was financially supported by the Slovenian Ministry of Higher Education, Science and Technology and the Ministry of Agriculture, Forestry and Food, Research programme P4-133; Project Number L7-7602 and V4-0484.

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Correspondence to Maja Ivana Smodiš Škerl.

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Smodiš Škerl, M.I., Kmecl, V. & Gregorc, A. Exposure to Pesticides at Sublethal Level and Their Distribution Within a Honey Bee (Apis mellifera) Colony. Bull Environ Contam Toxicol 85, 125–128 (2010). https://doi.org/10.1007/s00128-010-0069-y

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  • DOI: https://doi.org/10.1007/s00128-010-0069-y

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