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Dimethylarsinous Acid Disturbs Cytokinesis

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Abstract

Dimethylarsinous acid (DMAIII) induced tetraploidy and multinucleation in V79 cells. While the highest yield of tetraploids and multinucleated cells was at 1.25 μM of DMAIII, the mitotic index was highest at DMAIII 2.5 μM due to mitotic arrest. Mitosis was not observed at 5 μM. We observed V79 cells treated with DMAIII and visualized with rhodamine-phalloidin. Abnormal actin location was observed in the dividing cells which were treated with DMAIII and visualized with rhodamine-phalloidin. These findings suggested strongly that DMAIII inhibits not only formation of the normal mitotic spindle but cytokinesis and induces the formation of multinuclear cells.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, School of Human Environmental Science, Mukogawa Women’s University, 6-46 Ikebiraki-cho, Nishinomiya, 663-8558, Japan

    Mari Kitamura & Koichi Kuroda

  2. Department of Preventive Medicine and Environmental Health, Osaka City University Medical School, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan

    Ginji Endo

Authors
  1. Mari Kitamura
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  2. Koichi Kuroda
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  3. Ginji Endo
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Correspondence to Mari Kitamura.

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Kitamura, M., Kuroda, K. & Endo, G. Dimethylarsinous Acid Disturbs Cytokinesis. Bull Environ Contam Toxicol 83, 15–18 (2009). https://doi.org/10.1007/s00128-009-9709-5

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  • DOI: https://doi.org/10.1007/s00128-009-9709-5

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