Abstract
Glutathione S-transferase (GST) in the hepatopancreas of crustaceans has been suggested as a biomarker for organic pollution. However, much of crustacean physiology is known to exhibit a cyclic characteristic because of the periodic shedding of the confining exoskeleton. The goal of this study was to determine whether hepatopancreatic GST activity varies during the molting cycle using the fiddler crab, Uca pugilator, as the model. Neither the molting cycle nor 20-hydroxyecdysone injection had a significant effect on hepatopancreatic GST activity, suggesting GST activity is not under control of the molting hormone in Uca pugilator.
Similar content being viewed by others
References
Arun S, Rajendran A, Subramanian P (2006) Subcellular/tissue distribution and response to oil exposure of the cytochrome P40-dependent monoxygenase system and glutathione S-transferase in freshwater prawns. Exotoxicology 15:341–346. doi:10.1007/s10646-006-0074-4
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3
Drach P (1939) Mue et cycle d’intermue chez les Crustacés Décapodes. Ann Inst Océanogr Monaco 19:103–391
Eickhoff CV, He S-X, Gobas FAPC, Law FCP (2003) Determination of polycyclic aromatic hydrocarbons in dungeness crabs (Cancer magister) near an aluminum smelter in Kitimat Arm, British Columbia, Canada. Environ Toxicol Chem 22:50–58. doi :10.1897/1551-5028(2003)022<0050:DOPAHI>2.0.CO;2
Fernandez Gimenez AV, Garcia-Carreno FL, Navarrete del Toro MA, Fenucci JL (2002) Digestive proteinases of Artemesia longinaris (Decapoda, Penaeidae) and relationship with molting. Comp Biochem Physiol 132B:593–598
Gowland BTG, Moffat CF, Stagg RM, Houlihan DF, Davies IM (2002) Cypermethrin induces glutathione S-transferase activity in the shore crab, Carcinus maenas. Mar Environ Res 54:169–177. doi:10.1016/S0141-1136(02)00105-8
Ishizuka M, Sakiyama T, Iwata H, Fukushima M, Kazusaka A, Fujita S (1998) Accumulation of halogenated aromatic hydrocarbons and activities of cytochrome P450 and glutathione S-transferase in crabs (Eriocheir japonicus) from Japanese rivers. Environ Toxicol Chem 17:1490–1498. doi :10.1897/1551-5028(1998)017<1490:AOHAHA>2.3.CO;2
McLoughlin N, Yin D, Maltby L, Wood RM, Yu H (2000) Evaluation of sensitivity and specificity of two crustacean biochemical biomarkers. Environ Toxicol Chem 19:2085–2092. doi :10.1897/1551-5028(2000)019<2085:EOSASO>2.3.CO;2
Menone ML, Bortulos A, Botto F, Aizpun de Moreno JE, Moreno VJ, Iribarne O, Metcalfe TL, Metcalfe CD (2000) Organochlorine contaminants in a coastal lagoon in Argentina: analysis of sediment, crabs, and cordgrass from two different habitats. Estuaries 23:583–592. doi:10.2307/1353148
Vega-Villasante F, Fernandez I, Preciado RM, Oliva M, Tovar D, Nolasco H (1999) The activity of digestive enzymes during the molting stages of the arched swimming Callinectes arcuatus Ordway, 1863 (Crustacea: Decapoda: Portunidae). Bull Mar Sci 65:1–9
Vigh DA, Fingerman M (1985) Molt staging in the fiddler crab Uca pugilator. J Crust Biol 5:386–396. doi:10.2307/1547910
Zilli L, Schiavone R, Scordella G, Zonno V, Verri T, Storelli C, Vilella S (2003) Changes in cell type composition and enzymatic activities in the hepatopancreas of Marsupenaeu japonicus during the moulting cycle. J Comp Physiol 173B:355–363
Zou E, Fingerman M (1999a) Chitobiase activity in the epidermis and hepatopancreas of the fiddler crab Uca pugilator during the molting cycle. Mar Biol 133:97–101. doi:10.1007/s002270050447
Zou E, Fingerman M (1999b) Patterns of N-acetyl-β-glucosaminidase isoenzymes in the epidermis and hepatopancreas and induction of N-acetyl-β-glucosaminidase activity by 20-hydroxyecdysone in the fiddler crab, Uca pugilator. Comp Biochem Physiol 124C:345–349
Acknowledgments
Appreciation is expressed to Ms. Yanling Meng for her technical assistance. This investigation was supported by grant LEQSF (2005-08)-RD-A-26 from the Louisiana Board of Regents. We wish to thank the anonymous reviewers for suggestions that lead to the improvement of this manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hotard, S., Zou, E. Activity of Glutathione S-Transferase in the Hepatopancreas is not Influenced by the Molting Cycle in the Fiddler Crab, Uca pugilator . Bull Environ Contam Toxicol 81, 242–244 (2008). https://doi.org/10.1007/s00128-008-9487-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-008-9487-5