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Mapping and characterization of the new adult plant leaf rust resistance gene Lr77 derived from Santa Fe winter wheat

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Abstract

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A new gene for adult plant leaf rust resistance in wheat was mapped to chromosome 3BL. This gene was designated as Lr77.

Abstract

‘Santa Fe’ is a hard red winter cultivar that has had long-lasting resistance to the leaf rust fungus, Puccinia triticina. The objective of this study was to determine the chromosome location of the adult plant leaf rust resistance in Santa Fe wheat. A partial backcross line of ‘Thatcher’ (Tc) wheat with adult plant leaf rust resistance derived from Santa Fe was crossed with Thatcher to develop a Thatcher//Tc*2/Santa Fe F6 recombinant inbred line (RIL) population. The RIL population and parental lines were evaluated for segregation of leaf rust resistance in three field plot tests and in an adult plant greenhouse test. A genetic map of the RIL population was constructed using 90,000 single-nucleotide polymorphism (SNP) markers with the Illumina Infinium iSelect 90K wheat bead array. A significant quantitative trait locus for reduction of leaf rust severity in all four tests was found on chromosome 3BL that segregated as a single adult plant resistance gene. The RILs with the allele from the resistant parent for SNP marker IWB10344 had lower leaf rust severity and a moderately resistant to moderately susceptible response compared to the susceptible RILs and Thatcher. The gene derived from Santa Fe on chromosome 3BL was designated as Lr77. Kompetitive allele-specific polymerase chain reaction assay markers linked to Lr77 on 3BL should be useful for selection of wheat germplasm with this gene.

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Acknowledgements

We thank K. Xiao for excellent technical assistance.

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Authors and Affiliations

  1. Cereal Disease Laboratory, Agricultural Research Service, United States Department of Agriculture, St. Paul, MN, 55108, USA

    James A. Kolmer

  2. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Zhenqi Su & Amy Bernardo

  3. Hard Red Winter Wheat Genetics Research, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS, 66506, USA

    Guihua Bai

  4. Cereal Crops Research Unit, Agricultural Research Service, United States Department of Agriculture, Fargo, ND, 58102, USA

    Shiaoman Chao

Authors
  1. James A. Kolmer
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  2. Zhenqi Su
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  3. Amy Bernardo
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  4. Guihua Bai
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  5. Shiaoman Chao
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Correspondence to James A. Kolmer.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Communicated by Evans Lagudah.

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Kolmer, J.A., Su, Z., Bernardo, A. et al. Mapping and characterization of the new adult plant leaf rust resistance gene Lr77 derived from Santa Fe winter wheat. Theor Appl Genet 131, 1553–1560 (2018). https://doi.org/10.1007/s00122-018-3097-3

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  • DOI: https://doi.org/10.1007/s00122-018-3097-3

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