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A novel gene IBF1 is required for the inhibition of brown pigment deposition in rice hull furrows

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Abstract

The role of flavonoids as the major red, blue, purple and brown pigments in plants has gained these secondary products a great deal of attention over the years. In this study, we characterized a rice inhibitor for brown furrows1 (ibf1) mutant. In the ibf1 mutant, brown pigments specifically accumulate in hull furrows during seed maturation and reach a maximum level in dry seeds. Higher amounts of total flavonoids and anthocyanin in hull may be responsible for the brown pigmentation of ibf1. The IBF1 gene, which encodes a similar kelch repeat-containing F-box protein, was isolated by map-based cloning approach. Real-time RT-PCR and GUS activity assays revealed that IBF1 specifically expressed in reproductive tissues. GFP–IBF1 fusion protein mainly localized in cytoplasm. The expression of some major structural enzymatic genes involved in flavonoids biosynthesis could be up- or down-regulated to some different extent in ibf1 mutant. Our data suggested that IBF1 as a suppressor could inhibit the brown pigmentation of rice hull furrows.

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Acknowledgments

This work was supported by grants from the Ministry of Sciences and Technology of China (2011ZX08009-006 and 2009CB118500) and the National Natural Science Foundation of China (30760100 and 31160223).

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Tian Shao, Ding Tang, Ming Li & Zhukuan Cheng

  2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China

    Qian Qian

  3. Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Key Laboratory of Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming, 650201, China

    Jun Chen & Qiong Luo

Authors
  1. Tian Shao
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  2. Qian Qian
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  3. Ding Tang
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  4. Jun Chen
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  5. Ming Li
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  6. Zhukuan Cheng
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  7. Qiong Luo
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Corresponding authors

Correspondence to Zhukuan Cheng or Qiong Luo.

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Communicated by M. Xu.

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Shao, T., Qian, Q., Tang, D. et al. A novel gene IBF1 is required for the inhibition of brown pigment deposition in rice hull furrows. Theor Appl Genet 125, 381–390 (2012). https://doi.org/10.1007/s00122-012-1840-8

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  • DOI: https://doi.org/10.1007/s00122-012-1840-8

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