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Transcriptional responses of Italian ryegrass during interaction with Xanthomonas translucens pv. graminis reveal novel candidate genes for bacterial wilt resistance

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Abstract

Xanthomonas translucens pv. graminis (Xtg) causes bacterial wilt, a severe disease of forage grasses such as Italian ryegrass (Lolium multiflorum Lam.). In order to gain a more detailed understanding of the genetic control of resistance mechanisms and to provide prerequisites for marker assisted selection, the partial transcriptomes of two Italian ryegrass genotypes, one resistant and one susceptible to bacterial wilt were compared at four time points after Xtg infection. A cDNA microarray developed from a perennial ryegrass (Lolium perenne) expressed sequence tag set consisting of 9,990 unique genes was used for transcriptome analysis in Italian ryegrass. An average of 4,487 (45%) of the perennial ryegrass sequences spotted on the cDNA microarray were detected by cross-hybridisation to Italian ryegrass. Transcriptome analyses of the resistant versus the susceptible genotype revealed substantial gene expression differences (>1,200) indicating that great gene expression differences between different Italian ryegrass genotypes exist which potentially contribute to the observed phenotypic divergence in Xtg resistance between the two genotypes. In the resistant genotype, several genes differentially expressed after Xtg inoculation were identified which revealed similarities to transcriptional changes triggered by pathogen-associated molecular patterns in other plant–pathogen interactions. These genes represent candidate genes of particular interest for the development of tools for marker assisted resistance breeding.

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Acknowledgments

This research was funded by the Swiss National Science Foundation SNF Project (3100A0-112582). We would like to thank L. B. Jensen, B. Studer and L. B. Holte for technical and personal assistance in the department of Genetics and Biotechnology at Det Jorbrugvidenskabelige Fakultet (DJF), University of Aarhus. Special thanks are extended to S. Reinhard and P. Streckeisen for technical assistance at Agroscope Reckenholz-Tänikon, H.-P. Piepho and A. Schützenmeister for help with the experimental design and H. Rehrauer from the Functional Genomics Center Zürich for assistance with the statistical analyses.

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  1. Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, 8046, Zurich, Switzerland

    Fabienne Wichmann, Franco Widmer & Roland Kölliker

  2. Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Research Centre Flakkebjerg, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark

    Torben Asp

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  1. Fabienne Wichmann
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  2. Torben Asp
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  3. Franco Widmer
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  4. Roland Kölliker
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Correspondence to Roland Kölliker.

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Communicated by T. Luebberstedt.

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Wichmann, F., Asp, T., Widmer, F. et al. Transcriptional responses of Italian ryegrass during interaction with Xanthomonas translucens pv. graminis reveal novel candidate genes for bacterial wilt resistance. Theor Appl Genet 122, 567–579 (2011). https://doi.org/10.1007/s00122-010-1470-y

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