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Mapping of genome-wide resistance gene analogs (RGAs) in maize (Zea mays L.)

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Abstract

Isolation and mapping of genome-wide resistance (R) gene analogs (RGAs) is of importance in identifying candidate(s) for a particular resistance gene/QTL. Here we reported our result in mapping totally 228 genome-wide RGAs in maize. By developing RGA-tagged markers and subsequent genotyping a population consisting of 294 recombinant inbred lines (RILs), 67 RGAs were genetically mapped on maize genome. Meanwhile, in silico mapping was conducted to anchor 113 RGAs by comparing all 228 RGAs to those anchored EST and BAC/BAC-end sequences via tblastx search (E-value < 10−20). All RGAs from different mapping efforts were integrated into the existing SSR linkage map. After accounting for redundancy, the resultant RGA linkage map was composed of 153 RGAs that were mapped onto 172 loci on maize genome, and the mapped RGAs accounted for approximate three quarters of the genome-wide RGAs in maize. The extensive co-localizations were observed between mapped RGAs and resistance gene/QTL loci, implying the usefulness of this RGA linkage map in R gene cloning via candidate gene approach.

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Acknowledgments

This study was financially supported by the national outstanding youth foundation of China, ‘Construction of a linkage map for genome-wide resistance gene analogs and isolation of important genes in maize’, Grant No. 30525035; the national ‘863’ high-tech program of China, ‘Functional genomics on resistance to diseases, tolerance to abiotic stress, and grain quality in maize’, Grant No. 2006AA10A107; and the natural scientific foundation of Beijing, Grant No. 5060001.

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Authors and Affiliations

  1. National Maize Improvement Center of China, China Agricultural University, 2 west Yuanmingyuan Road, Beijing, 100094, People’s Republic of China

    Wenkai Xiao, Jing Zhao, Shengci Fan, Lin Li, Jinrui Dai & Mingliang Xu

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  1. Wenkai Xiao
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  2. Jing Zhao
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  3. Shengci Fan
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  4. Lin Li
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  5. Jinrui Dai
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  6. Mingliang Xu
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Correspondence to Mingliang Xu.

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Communicated by T. Lübberstedt.

Wenkai Xiao, Jing Zhao and Shengci Fan have contributed equally to this research.

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ESM1 (DOC 248 kb)

122_2007_583_MOESM2_ESM.doc

Supplementary Table 1 A summarization of the 228 genome-wide maize RGAs, including the known resistance genes used for data-mining maize EST databases, R-gene-like unigene/EST, RGA IDs and their chromosomal locations, and mapping approaches used in the present study (DOC 581 kb)

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Xiao, W., Zhao, J., Fan, S. et al. Mapping of genome-wide resistance gene analogs (RGAs) in maize (Zea mays L.). Theor Appl Genet 115, 501–508 (2007). https://doi.org/10.1007/s00122-007-0583-4

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  • DOI: https://doi.org/10.1007/s00122-007-0583-4

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