Abstract
To increase the value of associated molecular tools and also to begin to explore the degree to which interspecific and intraspecific genetic variation in Sorghum is attributable to corresponding genetic loci, we have aligned genetic maps derived from two sorghum populations that share one common parent (Sorghum bicolor L. Moench accession BTx623) but differ in morphological and evolutionarily distant alternate parents (S. propinquum or S. bicolor accession IS3620C). A total of 106 well-distributed DNA markers provide for map alignment, revealing only six nominal differences in marker order that are readily explained by sampling variation or mapping of paralogous loci. We also report a total of 61 new QTLs detected from 17 traits in these crosses. Among eight corresponding traits (some new, some previously published) that could be directly compared between the two maps, QTLs for two (tiller height and tiller number) were found to correspond in a non-random manner (P<0.05). For several other traits, correspondence of subsets of QTLs narrowly missed statistical significance. In particular, several QTLs for leaf senescence were near loci previously mapped for ‘stay-green’ that have been implicated by others in drought tolerance. These data provide strong validation for the value of molecular tools developed in the interspecific cross for utilization in cultivated sorghum, and begin to separate QTLs that distinguish among Sorghum species from those that are informative within the cultigen (S. bicolor).
References
Agrama A, Widle E, Reese C, Campbell R, Tuinstra R (2002) Genetic mapping of QTLs associated with greenbug resistance and tolerance in Sorghum bicolor. Theor Appl Genet 104:1373–1378
Bhattramakki D, Dong J, Chhabra AK, Hart GE (2000) An integrated SSR and RFLP linkage map of Sorghum bicolor (L.) Moench. Genome 43:988–1002
Bowers JE, Abbey C, Anderson S, Chang C, Draye X, Hoppe AH, Jessup R, Lemke C, Lennington J, Li Z, Lin YR, Liu SC, Luo L, Marler BS, Ming R, Mitchell SE, Qiang D, Reischmann K, Schulze SR, Skinner DN, Wang YW, Kresovich S, Schertz KF, Paterson AH (2003) A high-density genetic recombination map of sequence-tagged sites for sorghum, as a framework for comparative structural and evolutionary genomics of tropical grains and grasses. Genetics 165:367–386
Crasta OR, Xu WW, Rosenow DT, Mullet J, Nguyen HT (1999) Mapping of post-flowering drought resistance traits in grain sorghum: association between QTLs influencing premature senescence and maturity. Mol Gen Genet 262:579–588
George ML, Prasanna BM, Rathore RS, Setty TA, Kasim F, Azrai M, Vasal S, Balla O, Hautea D, Canama A, Regalado E, Vargas M, Khairallah M, Jeffers D, Hoisington D (2003) Identification of QTLs conferring resistance to downy mildews of maize in Asia. Theor Appl Genet 107:544–551
Hart GE, Schertz KF, Peng Y, Syed NH (2001) Genetic mapping of Sorghum bicolor (L.) Moench QTLs that control variation in tillering and other morphological characters. Theor Appl Genet 103:1232–1242
Haussmann BI, Mahalakshmi V, Reddy BV, Seetharama N, Hash CT, Geiger HH (2002) QTL mapping of stay-green in two sorghum recombinant inbred populations. Theor Appl Genet 106:133–142
Katsar CS, Paterson RH, Teetes GL, Peterson GC (2002) Molecular analysis of sorghum resistance to the greenbug (Homoptera: Aphididae). J Econ Entomol 95:448–457
Kim JS, Klein PE, Klein RR, Price HJ, Mullet JE, Stelly DM (2005) Chromosome identification and nomenclature of Sorghum bicolor. Genetics 169:955–965
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Larsen RJ, Marx ML (1985) An introduction to probability and its applications. Prentice-Hall, Inc, Englewood Cliffs
Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D, Wang X, Liu X, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J (2003) Control of tillering in rice. Nature 422:618–621
Lin YR, Schertz KF, Paterson AH (1995) Comparative analysis of QTLs affecting plant height and maturity across the Poaceae, in reference to an interspecific sorghum population. Genetics 141:391–411
Menz MA, Klein RR, Mullet JE, Obert JA, Unruh NC, Klein PE (2002) A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers. Plant Mol Biol 48:483–499
Ming R, Del Monte TA, Hernandez E, Moore PH, Irvine JE, Paterson AH (2002) Comparative analysis of QTLs affecting plant height and flowering among closely-related diploid and polyploid genomes. Genome 45:794–803
Noor MA, Cunningham AL, Larkin JC (2001) Consequences of recombination rate variation on quantitative trait locus mapping studies. Simulations based on the Drosophila melanogaster genome. Genetics 159:581–588
Paterson AH, Lin YR, Li Z, Schertz KF, Doebley JF, Pinson SRM, Liu SC, Stansel JW, Irvine JE (1995a) Convergent domestication of cereal crops by independent mutations at corresponding genetic loci. Science 269(5231):1712–1718
Paterson AH, Schertz KF, Lin YR, Liu SC, Chang YL (1995b) The weediness of wild plants: molecular analysis of genes influencing dispersal and persistence of johnsongrass, Sorghum halepense (L.). Proc Natl Acad Sci USA 92:6127–6131
Sanchez AC, Subudhi PK, Rosenow DT, Nguyen HT (2002) Mapping QTLs associated with drought resistance in sorghum (Sorghum bicolor L. Moench). Plant Mol Biol 48:713–726
Tao YZ, Henzell RG, Jordan DR, Butler DG, Kelly AM, McIntyre CL (2000) Identification of genomic regions associated with stay green in sorghum by testing RILs in multiple environments. Theor Appl Genet 100:1225–1232
Xu W, Subudhi PK, Crasta OR, Rosenow DT, Mullet JE, Nguyen HT (2000) Molecular mapping of QTLs conferring stay-green in grain sorghum (Sorghum bicolor L. Moench). Genome 43:461–469
Acknowledgments
USDA National Research Initiative (A.H.P., K.F.S.), US National Science Foundation (A.H.P., P.E.K.), and USDA-ARS (K.F.S.).
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by F. Salamini
K.F. Schertz: deceased
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Feltus, F.A., Hart, G.E., Schertz, K.F. et al. Alignment of genetic maps and QTLs between inter- and intra-specific sorghum populations. Theor Appl Genet 112, 1295–1305 (2006). https://doi.org/10.1007/s00122-006-0232-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-006-0232-3