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Quantitative trait loci for cell wall components in recombinant inbred lines of maize (Zea mays L.) II: leaf sheath tissue

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Abstract

While maize silage is a significant feed component in animal production operations, little information is available on the genetic bases of fiber and lignin concentrations in maize, which are negatively correlated with digestibility. Fiber is composed largely of cellulose, hemicellulose and lignin, which are the primary components of plant cell walls. Variability for these traits in maize germplasm has been reported, but the sources of the variation and the relationships between these traits in different tissues are not well understood. In this study, 191 recombinant inbred lines of B73 (low-intermediate levels of cell wall components, CWCs) × De811 (high levels of CWCs) were analyzed for quantitative trait loci (QTL) associated with CWCs in the leaf sheath. Samples were harvested from plots at two locations in 1998 and one in 1999 and assayed for neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL). QTL were detected on all ten chromosomes, most in tissue specific clusters in concordance with the high genotypic correlations for CWCs within the same tissue. Adjustment of NDF for its subfraction, ADF, revealed that most of the genetic variation in NDF was probably due to variation in ADF. The low to moderate genotypic correlations for the same CWC across leaf sheath and stalk tissues indicate that some genes for CWCs may only be expressed in certain tissues. Many of the QTL herein were detected in other populations, and some are linked to candidate genes for cell wall carbohydrate biosynthesis.

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Acknowledgement

Research conducted in partial fulfillment of the Ph.D. degree by M. D. Krakowsky. This journal paper of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project No. 3134, was supported by Hatch Act and State of Iowa funds and The R. F. Baker Center for Plant Breeding.

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Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Tifton, GA, 31794, USA

    M.D. Krakowsky

  2. Department of Agronomy, Iowa State University, Ames, IA, 50011, USA

    M. Lee

  3. Department of Agronomy, University of Wisconsin, Madison, WI, 53706, USA

    J.G. Coors

Authors
  1. M.D. Krakowsky
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  2. M. Lee
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  3. J.G. Coors
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Correspondence to M.D. Krakowsky.

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Communicated by D. Hoisington

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Krakowsky, M., Lee, M. & Coors, J. Quantitative trait loci for cell wall components in recombinant inbred lines of maize (Zea mays L.) II: leaf sheath tissue. Theor Appl Genet 112, 717–726 (2006). https://doi.org/10.1007/s00122-005-0175-0

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  • DOI: https://doi.org/10.1007/s00122-005-0175-0

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