Abstract
We have used AFLPs to construct a genetic linkage map on a pseudo-F2 population of arabica coffee (Coffea arabica L.) derived from a cross between the cultivars Mokka hybrid and Catimor. Sixty trees from this population were selected on the basis of plant height distribution to construct a linkage map. A total of 456 dominant markers and eight co-dominant markers were generated from 288 AFLP primer combinations. Of the total number of markers generated, 68% were from cv. Catimor, 30% from cv. Mokka hybrid, and 2% were co-dominant. This distribution suggests that the heterozygosity within the cv. Catimor sub-genomes was twice that within the cv. Mokka hybrid sub-genomes. Linkage groups were constructed using MAPMAKER version 3.0, resulting in 16 major linkage groups containing 4–21 markers, and 15 small linkage groups consisting of 2–3 linked markers each. The total length of the map was 1,802.8 cM, with an average distance of 10.2 cM between adjacent markers. This genetic map will serve as the framework for mapping QTL controlling source-sink traits in the same population.
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Acknowledgements
We thank Susan Ancheta for technical assistance, Andrew Paterson for constructive discussions, Leslie Poland for planting and tending the mapping population at the Kunia substation, and Melinda Moore, Zhiyong Liu, and Stephanie Whalen for reviewing the manuscript. This work was supported by a U.S. Department of Agriculture, Agricultural Research Service Cooperative Agreement (No. CA 58-5320-8-134) with the Hawaii Agriculture Research Center.
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Communicated by H.F. Linskens
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Pearl, H.M., Nagai, C., Moore, P.H. et al. Construction of a genetic map for arabica coffee. Theor Appl Genet 108, 829–835 (2004). https://doi.org/10.1007/s00122-003-1498-3
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DOI: https://doi.org/10.1007/s00122-003-1498-3