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Mitochondrial coupling factor 6 upregulation in hypertension-induced cardiac hypertrophy

Hochregulation von „mitochondrial coupling factor 6“ bei hypertonieinduzierter kardialer Hypertrophie

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Abstract

Background

Mitochondrial coupling factor 6 (CF6) is a constriction factor in cardiac hypertrophy, whose mechanisms are not fully understood.

Materials and methods

Here, we established cardiac hypertrophy models for feeding spontaneously hypertensive rats (SHRs) aged 10, 20, and 30 weeks. Hemodynamic monitoring was performed during the feeding program to ensure the success of the model.

Results

Cardiac hypertrophy, but not fibrosis, occurred in the 10-, 20-, and 30-week-old SHRs. No significant changes in CF6 gene expression were detected by RT-PCR in any of the SHR groups as compared with the control groups (p > 0.05). ELISA assessment showed that the CF6 protein level in the 20- and 30-week-old SHRs with cardiac hypertrophy was significantlyincreased (vs. control, p < 0.05).

Conclusion

CF6 protein was upregulated in cardiac hypertrophy induced by hypertension; further mechanisms involved in this process should be investigated.

Zusammenfassung

Hintergrund

„Mitochondrial coupling factor 6“ (CF6) ist ein Konstriktionsfaktor bei kardialer Hypertrophie, dessen Wirkweise noch nicht vollständig bekannt ist.

Methoden

In der vorliegenden Arbeit entwickelten die Autoren Modelle kardialer Hypertrophie mit Fütterung spontan hypertensiver Ratten (SHR) im Alter von 10, 20 und 30 Wochen. Das hämodynamische Monitoring erfolgte im Rahmen des Fütterungsschemas, um den Erfolg des Modells zu sichern.

Ergebnisse

Es trat eine kardiale Hypertrophie, nicht aber eine Fibrose bei den 10, 20 und 30 Wochen alten SHR auf. Signifikante Veränderungen der CF6-Gen-Expression wurden mittels RT-PCR bei den 10, 20 und 30 Wochen alten SHR im Vergleich zu den jeweiligen Kontrollgruppen nicht festgestellt (p > 0,05). Die Untersuchung mittels ELISA ergab, dass der CF6-Protein-Wert bei den 20 und 30 Wochen alten SHR mit kardialer Hypertrophie (gegenüber den Kontrollen; p < 0,05) signifikant erhöht war.

Schlussfolgerung

Das CF6-Protein war bei durch Hypertonie induzierter kardialer Hypertrophie hochreguliert; weitere Abläufe, die an diesem Vorgang beteiligt sind, sollten noch untersucht werden.

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Acknowledgments

The project was supported by the National Natural Science Foundation of China (Grant No. 81200175), Qingdao Science and Technology Support Program [2011-2-3-2-(14)-nsh], and China Postdoctoral Science Foundation (2014M551872).

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Authors and Affiliations

  1. Key Laboratory of Cardiovascular Remodeling and Function, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, 250012, Jinan, Shandong, China

    Tao He &  Zhiming Ge

  2. Cardiology Department, Qingdao Municipal Hospital, 266033, Qingdao, Shandong, China

    Tao He, Aili Guan, Yue Shi &  Hongyan Dai

Authors
  1. Tao He
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  2. Aili Guan
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  3. Yue Shi
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  4. Zhiming Ge
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  5. Hongyan Dai
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Corresponding authors

Correspondence to Zhiming Ge or Hongyan Dai.

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Conflict of interest

T. He, A. Guan, Y. Shi, Z. Ge, and H. Dai state that there are no conflicts of interest.

All national guidelines on the care and use of laboratory animals have been followed and the necessary approval was obtained from the relevant authorities.

Additional information

T. He and A. Guan contributed equally to this work.

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He, T., Guan, A., Shi, Y. et al. Mitochondrial coupling factor 6 upregulation in hypertension-induced cardiac hypertrophy. Herz 40, 783–787 (2015). https://doi.org/10.1007/s00059-015-4301-8

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  • DOI: https://doi.org/10.1007/s00059-015-4301-8

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