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Substrate-based chemical probes for Alzheimer’s γ-secretase

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Abstract

The membrane-embedded γ-secretase complex carries out hydrolysis within the lipid bilayer in proteolyzing nearly 100 different membrane protein substrates. Among these substrates, the amyloid precursor protein (APP) has been the most studied, as generation of aggregation-prone amyloid β-peptide (Aβ) is a defining feature of Alzheimer’s disease (AD). Mutations in APP and in presenilin, the catalytic component of γ-secretase, cause familial AD, strong evidence for a pathogenic role of Aβ. However, in human trials γ-secretase inhibitors not only failed to slow AD progression, they worsened cognitive function. More in-depth study of γ-secretase and how AD-causing mutations alter its structure and function is clearly needed. Substrate-based chemical probes have been critical to unraveling the complexity of γ-secretase. Such synthetic peptides and peptidomimetics will be reviewed here, including recently reported structural and functional probes.

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Abbreviations

Alzheimer’s disease:

AD

α-aminoisobutyric acid:

Aib

Amyloid β-peptide:

Aβ

Amyloid precursor protein:

APP

Amyloid precursor protein intracellular domain:

AICD

99-residue APP membrane-bound stub:

C99

C-terminal fragment:

CTF

Familial Alzheimer’s disease:

FAD

Cryo-electron microscopy:

cryo-EM

Helical peptide inhibitor:

HPI

N-terminal fragment:

NTF

Presenilin-1:

PSEN1

Presenilin-2:

PSEN2

Transition-state analog inhibitor:

TSA

Transmembrane domain:

TMD

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Acknowledgements

This work was supported by grant GM122894 from the NIH.

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  1. Department of Medicinal Chemistry, University of Kansas, 1567 Irving Hill Road, GLH-2115, Lawrence, KS, 66045, USA

    Michael S. Wolfe

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Wolfe, M.S. Substrate-based chemical probes for Alzheimer’s γ-secretase. Med Chem Res 29, 1122–1132 (2020). https://doi.org/10.1007/s00044-020-02565-w

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