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Exploring inhibition mechanism and nature of lipase by Ligupurpuroside A extracted from Ku-Ding tea

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Abstract

Enzyme-activity essay demonstrated that Ligupurpuroside A acts as a natural inhibitor of lipase in a competitive manner. The inhibition mechanism and nature of lipase by Ligupurpuroside A were investigated by fluorescence spectra, UV–Vis absorption spectra, circular dichroism (CD) spectra and molecular docking methods. Fluorescence experiments indicated that Ligupurpuroside A can quench the intrinsic fluorescence of lipase through a static quenching procedure. Thermodynamic analysis suggested that hydrophobic interaction is the main force between lipase and Ligupurpuroside A. Fluorescence resonance energy transfer experiment showed that an energy transfer from lipase to Ligupurpuroside A occurs with great possibility, confirming the presence of static quenching mechanism of lipase fluorescence by Ligupurpuroside A. Interestingly, conformation of lipase remained almost the same after bound to Ligupurpuroside A based on CD spectral experiments. All these experimental results were validated by the protein-ligand docking studies which further showed that Ligupurpuroside A could bind to the amino acid residues of the hydrophobic cavity on catalytic sites of lipase. This study should be helpful for the design of diet drug and the full use of Ligupurpuroside A in food industry.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant 31540012, 31470431, 30570421, 31670360, 81501213), Guangdong Natural Science Foundation for Major cultivation project (2014A030308017, 2016A030313051), Shenzhen Science and Technology Innovation Committee Grants (JSGG20160229120821300, JCYJ20150625103526744, JCYJ20170302144535707, JSGG20130411160539208, CXZZ20150601110000604, ZDSYS201506031617582, KQCX20140522111508785, CXZZ20150529165110750) and Shenzhen special funds for Bio-industry development (NYSW20140327010012).

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    Authors and Affiliations

    1. Shenzhen Key Laboratory of Marine Bioresources and Ecology/Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, 518055, Shenzhen, China

      Yu Fan, Yang Xu, Qing-Guo Han, Liang-Liang Shen, Hong Xu, Zhi-Bing Wu, Xu Xu, Ming Ying & Zhang-Li Hu

    2. Shenzhen High School, 518001, Shenzhen, China

      Yang Xu

    3. Key Laboratory of RF Circuits and Systems of Ministry of Education, Hangzhou Dianzi University, 310018, Hangzhou, China

      Hong Xu

    4. School of Medicine, Shenzhen University, 518060, Shenzhen, China

      Zhen-Dan He

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    1. Yu Fan
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    Correspondence to Hong Xu or Zhang-Li Hu.

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    These authors contributed equally: Yu Fan, Yang Xu and Qing-Guo Han.

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    Fan, Y., Xu, Y., Han, QG. et al. Exploring inhibition mechanism and nature of lipase by Ligupurpuroside A extracted from Ku-Ding tea. Med Chem Res 27, 1822–1833 (2018). https://doi.org/10.1007/s00044-018-2194-9

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    • DOI: https://doi.org/10.1007/s00044-018-2194-9

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