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Trypsin inhibitory potential and microbial transformation of rutin isolated from Citrus sinensis

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Abstract

This study was designed to determine the trypsin inhibitory potential of rutin (1) and its biotransformed metabolite, quercetin (2). Rutin (1) was purified from the methanolic extract of Citrus sinensis and biotransformed in the culture of Streptomyces griseus, which yielded quercetin (2) as the major product. Both 1 and 2 showed significant trypsin inhibitory potential with IC50 = 0.016 ± 0.002 and 0.019 ± 0.003 mM, respectively. Kinetic studies were carried out using GraFit 7.0 software. Lineweaver–Burk and Dixon plots and their secondary replots indicated that these compounds are non-competitive inhibitors. The K i values of 1 and 2 were found to be 17.5 ± 0.7 and 3.1 ± 0.5 μM, respectively.

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Acknowledgments

The authors are grateful to Higher Education Commission of Pakistan for providing funds under Indigenous Scholarship Scheme (042-111212-200-PS2-200).

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Authors and Affiliations

  1. Research Lab II, Department of Chemistry, Government College University, Lahore, Pakistan

    Muhammad Asam Raza & Durre Shahwar

  2. Department of Chemistry, University of Gujrat, Hafiz Hayat Campus, Gujrat, Pakistan

    Muhammad Asam Raza

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  1. Muhammad Asam Raza
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  2. Durre Shahwar
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Correspondence to Muhammad Asam Raza.

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Raza, M.A., Shahwar, D. Trypsin inhibitory potential and microbial transformation of rutin isolated from Citrus sinensis . Med Chem Res 22, 3698–3702 (2013). https://doi.org/10.1007/s00044-012-0379-1

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  • DOI: https://doi.org/10.1007/s00044-012-0379-1

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