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3D QSAR studies on 5-(2-methylbenzimidazol-1-yl)-N-alkylthiophene-2-carboxamide derivatives as P. falciparum dihydroorotate dehydrogenase (PfDHODH) inhibitors

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Abstract

Malaria is currently one of the world’s most severe endemic diseases, responsible for majority of morbidity and mortality. A large number of drugs are available for its treatment; however, the development of resistance has become more widespread with most of the frontline drug therapies. Inhibitors of PfDHODH have proven efficacy for the treatment of malaria. 3D QSAR studies on some 5-(2-methylbenzimidazol-1-yl)-N-alkylthiophene-2-carboxamide derivatives as PfDHODH inhibitors were performed by comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) methods to rationalize the structural requirements responsible for the inhibitory activity of these compounds. The alignment strategy was used for these compounds by means of Distill function defined in SYBYL x 1.2. The best CoMFA and CoMSIA models obtained for the training set were statistically significant with q 2 of 0.669 and 0.727, cross-validated coefficient (r 2 cv) of 0.603 and 0.698, and conventional coefficients (r 2) of 0.971 and 0.966, respectively. Both the models were validated by an external test set of five compounds giving satisfactory prediction (r 2 pred) of 0.799 and 0.815 for CoMFA and CoMSIA models, respectively. Further the robustness of the model was verified by bootstrapping analysis. Generated CoMFA and CoMSIA models provide useful information for the design of novel inhibitors with better PfDHODH inhibitory activity.

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Acknowledgments

The authors would like to thank Director General, Nirma University, Ahmedabad, India for providing the computational facility to complete this work.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, Institute of Pharmacy, Nirma University, S.G. Highway, Chharodi, Ahmedabad, 382 481, Gujarat, India

    Vivek K. Vyas, Harshang Parikh & Manjunath Ghate

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  1. Vivek K. Vyas
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  2. Harshang Parikh
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  3. Manjunath Ghate
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Correspondence to Vivek K. Vyas.

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Vyas, V.K., Parikh, H. & Ghate, M. 3D QSAR studies on 5-(2-methylbenzimidazol-1-yl)-N-alkylthiophene-2-carboxamide derivatives as P. falciparum dihydroorotate dehydrogenase (PfDHODH) inhibitors. Med Chem Res 22, 2235–2243 (2013). https://doi.org/10.1007/s00044-012-0216-6

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