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DNA microarray-based gene expression profiling of estrogenic chemicals

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Abstract

We summarize updated information about DNA microarray-based gene expression profiling by focusing on its application to estrogenic chemicals. First, estrogenic chemicals, including natural/industrial estrogens and phytoestrogens, and the methods for detection and evaluation of estrogenic chemicals were overviewed along with a comprehensive list of estrogenic chemicals of natural or industrial origin. Second, gene expression profiling of chemicals using a focused microarray containing estrogen-responsive genes is summarized. Third, silent estrogens, a new type of estrogenic chemicals characterized by their estrogenic gene expression profiles without growth stimulative or inhibitory effects, have been identified so far exclusively by DNA microarray assay. Lastly, the prospect of a microarray assay is discussed, including issues such as commercialization, future directions of applications and quality control methods.

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Acknowledgments

This research has been supported partly by a Special Coordination Fund for Promoting Science and Technology (Encouraging Development of Strategic Research Centers), a Knowledge Cluster Initiative program and a Grant-in-aid for Basic Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by the Guideline Program for Medical Device Development from the Ministry of Economy, Trade and Industry of Japan.

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  1. Signaling Molecules Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    Ryoiti Kiyama & Yun Zhu

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Correspondence to Ryoiti Kiyama.

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Kiyama, R., Zhu, Y. DNA microarray-based gene expression profiling of estrogenic chemicals. Cell. Mol. Life Sci. 71, 2065–2082 (2014). https://doi.org/10.1007/s00018-013-1544-5

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