Abstract:
Cytochrome P450 1A (CYP1A) complementary DNA was isolated from eel (Anguilla japonica) liver treated with 3-methylcholanthrene. The cDNA contained a 5′ untranslated region of 163 bp, an open reading flame of 1560 bp coding for 519 amino acids and a stop codon, and a 3′ untranslated region of 1730 bp. The predicted molecular weight was approximately 58.4 kDa. The deduced amino acid sequence exhibited identities with reported CYP1A sequences of 80% for rainbow trout, 79% for scup, 76% for plaice and butterfly fish, and 74% for toadfish. When compared with mammalian CYP proteins, the eel CYP1A was more similar to CYP1A1 (54%–56%) than to CYP1A2 (49%–52%). Northern and Southern blot analyses showed two distinct bands, suggesting the existence of another 3-methylcholanthrene-inducible CYP1A gene in eel.
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Received December 19, 1998; accepted February 18, 1999
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Mitsuo, R., Itakura, T. & Sato, M. Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in Eel (Anguilla japonica). Mar. Biotechnol. 1, 353–358 (1999). https://doi.org/10.1007/PL00011786
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DOI: https://doi.org/10.1007/PL00011786