Abstract
Thyroglobulin (Tg) and its specific mRNA were analyzed in the thyroid gland of two cases of familial goiter. A three-fold increase in the level of stable protein and functional Tg mRNA was found in the goiter of one of the two patients in whom a desiodase defect was demonstrated. Normal concentrations of both Tg and its mRNA were found in the other goiter in which no enzymatic defect could be shown. Our results suggest that the increase of Tg mRNA levels found in the goiter with a desiodase defect was due to a chronic stimulation of the gland by TSH.
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Van Herle A.J., Vassart G., Dumont J.E. Control of thyroglobulin synthesis and secretion (first of two parts). N. Engl. J. Med. 301: 239, 1979.
Van Herle A.J., Vassart G., Dumont J.E. Control of thyroglobulin synthesis and secretion (second of two parts). N. Engl. J. Med. 301: 307, 1979.
Wager G.B., Lamberg A., Saarinen P. Congenital goiter with thyroglobulin deficiency. In: Robbins J., Braverman L.E. (Eds.), Thyroid research. Excerpta Medica, Amsterdam, 1976, p. 463.
Pammenter M., Bester A., Albrecht C., Van Jaarsveld P. Afrikander cattle congenital characteristics of the defective 19 S thyroglobulin synthesis. Endocrinology 104: 1853, 1979.
Tassi V.N., Di Lauro R., Van Jaarrsveld P., Alvino C.G. Two abnormal thyroglobulin like polypeptides produced from afrikander cattle congenital goiter mRNA. J. Biol. Chem. 259: 10505, 1984.
de Vijlder J.J.M., Van Ommen G.J.B., Van Voorthuizen W.F., Koch C.A.M., Anberg A.C., Vassart G., Dinsart C., Flavell R.A. Non functional thyroglobulin messanger RNA in goats with hereditary congenital goiter. J. Mol. Appl. Genet. 1: 51, 1981.
Cabrer B., Brocas H., Perez-Castillo A., Vassart G. Human congenital goiter with impaired thyroglobulin synthesis. Ann. Endocrinol. (Paris) 46: ---, 1982.
Colletta G., Pinto A., Di Fiore P.P., Fusco A., Ferrentino M., Avvedimento V.E., Tsuchida N., Vecchio G. Dissociation between transformed and differentiated phenotype in rat thyroid cells after transformation with a TS mutant of kirsten murine sarcoma virus. Mol. Cell. Biol. 3: 2099, 1983.
Gordon J.I., Deeley R.G., Burns A.T.H., Paterson B.M., Christmann J.L., Goldberg R.F. In vitro translation of avian vitellogenin messanger RNA. J. Biol. Chem. 252: 8310, 1977.
Alvino C.G., Tassi V., Patterson B.M., Di Lauro R. In vitro synthesis of 300.000 Mr rat thyroglobulin subunit. FEBS Lett. 137: 307, 1982.
Maniatis T., Fritsch E.F., Sambrook J. Electrophoresis of RNA through gels containing formaldehyde. In: Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, 1982, p. 202.
Thomas P.S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77: 5201, 1980.
Di Lauro R., Obici S., Acquaviva A.M., Alvino C.G. Construction of recombinant plasmids containing rat thyroglobulin mRNA. Gene 19: 117, 1982.
Rigby P.W.J., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113: 237, 1977.
Bergé-Lefranc J.L., Cartouzou G., Bigno C., Lissintzky S. Quantitative in situ hybridization of 3H-labeled complementary deoxyribonucleic acid (cDNA) to the messanger ribonucleic acid of thyroglobulin in human thyroid tissues. J. Clin. Endocrinol. Metab. 57: 470, 1983.
Uller R.P., Van Herle A.J., Chopra I.J. Comparison of alterations in circulating thyroglobulin, triiodothyronine and thyroxine in response to exogenous (bovine) and endogenous (human) thyrotropin. J. Clin. Endocrinol. Metab. 37: 741, 1973.
Torrigiani C., Doniach D., Roitt I.M. Serum thyroglobulin levels in healthy subjects and patients with thyroid disease. J. Clin. Endocrinol. Metab. 29: 305, 1969.
Pezzino V., Cozzani P., Filetti S. A radioimmunoassay for human thyroglobulin: methodology and clinical applications. Eur. J. Clin. Invest. 7: 503, 1977.
Pezzino V., Vigneri R., Squatrito S. Increased serum thyroglobulin levels in patients with nontoxic goiter. J. Clin. Endocrinol. Metab. 46: 653, 1978.
Avvedimento V.E., Tramontano D., Ursini M.V., Monticelli A., Di Lauro R. The level of thyroglobulin mRNA is regulated by TSH both in vitro and in vivo. Biochem. Biophys. Res. Commun. 122: 472, 1984.
Van Heuverswyn B., Streydio C., Brocas H., Refetoff S., Dumont J.E., Vassart G. Thyrotropin controls transcription of the thyroglobulin gene. Proc. Natl. Acad. Sci. USA 81: 5941, 1984.
Chebath G., Chabaud O., Mauchamp J. Modulation of thyroglobulin messenger RNA level by thyrotropin in cultured thyroid cells. Nucl. Acids Res. 6: 3352, 1979.
Bjorkmann U., Ekholm R., Ericson L.E. Effects of thyrotropin on thyroglobulin exocytosis and iodination in the rat thyroid gland. Endocrinology 102: 460, 1978.
Wollmann S.H. Secretion of thyroid hormones. In: Dingle J.T., Fell H.B. (Eds.), Lysosomes in biology and pathology. Amsterdam, North Holland Publishing Company, vol. 2, 1969, p. 483.
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Monticelli, A., Avvedimento, V.E., Mariano, A. et al. Increased level of thyroglobulin mRNA in a human familial goiter. J Endocrinol Invest 10, 59–63 (1987). https://doi.org/10.1007/BF03347155
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DOI: https://doi.org/10.1007/BF03347155