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Is prolactin playing a role in the regulation of catecholamine synthesis and release from male rat adrenal medulla?

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Abstract

Previous evidence allows one to suspect that prolactin (PRL) may be a physiological regulator of catecholamine (CA) synthesis and release in the adrenal gland of rodents. To explore this possibility, we studied the in vivo and in vitro metabolism and release of noradrenaline (NA) and adrenaline (A) in the adrenal gland of male rats. The study was carried out with animals exhibiting a moderate increase in plasma PRL levels induced by grafting of additional pituitaries or a severe hyperprolactinemia produced by diethylstilbestrol (DES)-induced pituitary hyperplasia. The latter animals exhibited a significant increase in adrenal weight, associated with decrease in tyrosine hydroxylase (TH) activity and in NA content. Moreover, the adrenal activity of phenylethanolamine-N-methyl transferease (PNMT) was decreased in DES-treated animals. Pituitary-grafted rats also displayed an increased adrenal weight, together with decreases in the activities of PNMT, catechol-O-methyl tansferase and monoamine oxidase. These in vivo observations were followed by in vitro studies, which showed a decrease in the basal release of both CAs from incubated adrenals of DES-treated rats, with no changes in pituitary-grafted rats. In addition, exposure to PRL of the incubated adrenals of animals exhibiting normal PRL levels produced decreases in A release and storage and in TH activity. These observations allow us to conclude that: i) PRL appears to exert an inhibitory influence on the catecholaminergic activity in the adrenal gland; and ii) its effect seems to be exerted by a direct action on this gland.

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References

  1. Ungar A., Phillips J.H. Regulation of the adrenal medulla. Physiol. Rev. 63: 787, 1983.

    PubMed  CAS  Google Scholar 

  2. Mizobe E., Kozousek V., Dean D.M., Livett B.G. Pharmacological characterization of adrenal paraneurons: substance P and somatostatin as inhibitory modulators of the nicotinic response. Brain. Res. 178: 555, 1979.

    Article  PubMed  CAS  Google Scholar 

  3. Okubo M., Kaku K., Kaneko T., Yanaihara N. Effect of bombesin and gastrin releasing peptide on catecholamine secretion from rat adrenal gland, in vitro. Endocrinol. Jpn. 32: 21, 1985.

    Article  PubMed  CAS  Google Scholar 

  4. Cheung C.Y., Holzwarth M.A. Fetal adrenal VIP: distribution and effect on adrenomedullary catecholamine secretion. Peptides 7: 413, 1986.

    Article  PubMed  CAS  Google Scholar 

  5. Fernández-Ruiz J.J., Cebeira M., Agrasal C., Tresguerres J.A.F., Esquifino A.I., Ramos J.A. Effect of elevated prolactin levels on the synthesis and release of catecholamine from the adrenal medulla in female rats. Neuroendocrinology 45: 208, 1987.

    Article  PubMed  Google Scholar 

  6. Bukhari A.R., Fernández-Ruiz J.J., Pais J.R., Alvarez C., Ramos J.A. Changes in catecholamine metabolism in adrenal medulla of adult male rats with experimentally induced hyperprolactinemia. Biog. Amin. 5: 103, 1988.

    Google Scholar 

  7. Fernández-Ruiz J.J., Esquifino A.I., Steger R.W., Bartke A., Hodges S.L. Effects of prolactin on the adrenal medulla in mice and hamsters. Neuroendocrinol. Let. 10: 5, 1988.

    Google Scholar 

  8. Fernández-Ruiz J.J., Esquifino A.I., Steger R.W., Bukhari A.R., Bartke A. Changes in the catecholamine metabolism in the adrenal medulla of male hamsters with experimental hyperprolactinemia. Comp. Biochem. Physiol. 93C: 303, 1989.

    Google Scholar 

  9. Lewinsky A., Bartke A., Esquifino A.I., Sewerynek E., Steger R.W. Adrenal catecholamine content: effect of congenital GH, PRL and TSH deficiency and of hormone replacement therapy in the male mouse. Exp. Clin. Endocrinol. 87: 176, 1986.

    Article  Google Scholar 

  10. Graham A.D.M., Longo L.D., Cheung C.Y. Catecholamine secretion from the adrenal medulla of the fetus, regulation by hormones. J. Develop. Physiol. 8: 227, 1986.

    CAS  Google Scholar 

  11. Barke A., Doherty P., Steger R.W., Morgan W.W., Amador A.G., Herbert D.C., Siler-Khodr T.M., Smith M.S., Klemcke H.G., Hymer W.C. Effects of estrogen-induced hyperprolactinemia on endocrine and sexual functions in adult male rats. Neuroendocrinology 39: 126, 1984.

    Article  Google Scholar 

  12. Adler R.A. The anterior pituitary-grafted rat: a valid model of chronic hyperprolactinemia. Endocr. Rev. 7: 302, 1986.

    Article  PubMed  CAS  Google Scholar 

  13. Fernández-Ruiz J.J., Bukhari A.R., Martìnez R., Tresguerres J.A.F., Ramos J.A. Effects of estrogens and progesterone on the catecholaminergic activity of the adrenal medulla in female rats. Life Sci. 42: 1019, 1988.

    Article  PubMed  Google Scholar 

  14. Wurtman R.J., Axelrod J.A. A sensitive and specific assay for the estimation of monoamine oxidase. Biochem. Pharmacol. 12: 1439, 1963.

    Article  PubMed  CAS  Google Scholar 

  15. Axelrod J.A. Purification and properties of PNMT. J. Biol. Chem. 237: 1657, 1962.

    PubMed  CAS  Google Scholar 

  16. Wurtman R.J., Axelrod J.A. Adrenaline syntesis: control by the pituitary gland and adrenal glucocorticoids. Science 150: 1464, 1965.

    Article  PubMed  CAS  Google Scholar 

  17. Parvez H., Parvez S. Microradioisotopic determination of enzymes COMT, PNMT and MAO in a single tissue homogenate. Clin. Chim. Acta 146: 85, 1973.

    Article  Google Scholar 

  18. Nagatsu T., Oka K., Kato T. Highly sensitive assay for tyrosine hydroxylase activity by high performance liquid chromatography. J. Chromatogr. 163: 247, 1979.

    Article  PubMed  CAS  Google Scholar 

  19. Fernández-Ruiz J.J., Esquifino A.I., Steger R.W., Amador A.G., Bartke A. Presence of tyrosine hydroxylase activity in anterior pituitary adenomas and ectopic anterior pituitaries in male rats. Brain Res. 421: 65, 1987.

    Article  PubMed  Google Scholar 

  20. Fernández-Ruiz J.J., Bukhari A.R., Hernández M.L., Alemany J., Ramos J.A. Sex- and age-dependent changes in catecholamine metabolism and release of rat adrenal gland. Neurobiol. Aging 10: 331, 1989.

    Article  PubMed  Google Scholar 

  21. Cryer P. Physiology and pathophysiology of the human sympathoadrenal neuroendocrine system. N. Engl. J. Med. 303: 426, 1980.

    Article  Google Scholar 

  22. Palmer S., Oakes G., Champion J., Fisher D.A., Hobel C. Catecholamine physiology in the ovine fetus. III. Maternal and fetal response to acute maternal exercise. Am. J. Obstet. Gynecol. 149: 426, 1984

    Article  PubMed  CAS  Google Scholar 

  23. Fernández-Ruiz J.J., Martínez R., Hernández M.L., Ramos J.A. Possible direct effect of prolactin on catecholamine syntesis and release in rat adrenal medulla: in vitro studies. J. Endocrinol. Invest. 11: 603, 1988.

    Article  PubMed  Google Scholar 

  24. Hernández M.L., de Miguel R.; Ramos J.A., Fernández-Ruiz J.J. Prolactin inhibits the activity of tyrosine hydroxylase in cultured bovine adrenal chromaffin cells in a dose-dependent manner. Brain Res. 528: 175, 1990.

    Article  PubMed  Google Scholar 

  25. Calvo J.C., Finocchiaro L., Luthy I., Charreau E.H., Calandra R.S., Engström B., Hansson V. Specific prolactin binding in the rat adrenal gland: its characterization and hormonal regulation. J. Endocrinol. 89: 317, 1981.

    Article  PubMed  CAS  Google Scholar 

  26. Marshall S., Huang H.H., Kledzik G.S., Campbell G., Meites J. Glucocorticoid regulation of prolactin receptors in kidneys and adrenals of male rats. Endocrinology 102: 869, 1978.

    Article  PubMed  CAS  Google Scholar 

  27. Katikinemi M., Davies T.F., Catt K.J. Regulation of adrenal and testicular prolactin receptors by adrenocorticotropic hormone and luteinizing hormone. Endocrinology 108: 2367, 1981.

    Article  Google Scholar 

  28. Klemcke H.G., Nienaber J. A role for prolactin in the porcine stress response. 69th Annual Meeting of the Endocrine Society, Indianapolis, IN, 1987, Abstract 341.

  29. George R.J., Haycock J., Johnston J.P., Craviso G.L., Waymire J.C. In vitro phosphorylation of bovine adrenal chromaffin cell tyrosine hydroxylase by endogenous protein kinase. J. Neurochem. 52: 274, 1989.

    Article  PubMed  CAS  Google Scholar 

  30. Pocotte S.L., Holz R.W. Effects of phorbol ester on tyrosine hydroxylase phosphorylation and activation in cultured bovine adrenal chromaffin cells. J. Biol Chem 261: 1873, 1986.

    PubMed  CAS  Google Scholar 

  31. Marshall S., Kledzik G.S., Gelato M., Campbell G., Meites J. Effects of estrogens and testosterone on specific prolactin binding in the kidneys and adrenals of rats. Steroids 27: 187, 1976.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Complutense University, 28040, Madrid, Spain

    M. L. Hernández, R. de Miguel, J. A. Ramos & J. J. Fernández-Ruiz

Authors
  1. M. L. Hernández
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  2. R. de Miguel
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  3. J. A. Ramos
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  4. J. J. Fernández-Ruiz
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Additional information

A preliminary report of this work has been presented and published in abstract form at the “III Congreso Hispano-Luso de Bioquímica, Santiago de Compostela, 1988”.

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Hernández, M.L., de Miguel, R., Ramos, J.A. et al. Is prolactin playing a role in the regulation of catecholamine synthesis and release from male rat adrenal medulla?. J Endocrinol Invest 14, 201–208 (1991). https://doi.org/10.1007/BF03346789

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