Abstract
Nitrate reductase activity in the leaves of a number of plants after transfer from light to dark was assayed both by in vivo and in vitro methods. The initial activity persisted during the dark phase for a considerable length of time and declined gradually. After exposure to light again, the NR activity increased rapidly. The possibility of nitrate assimilation in complete darkness is discussed.
Similar content being viewed by others
References
Abrol VP, Sawhney SK & Naik MS, Plant Cell Environ, 6 (1983) 595.
Naik MS & Nicholas DJD, Phytochemistry, 25 (1986) 571.
Oaks A, Plant Physiol, 106 (1994) 407.
Huber JL, Huber SC, Campbell WH & Redinbaugh MG, Arch Biochem Biophys, 296 (1992) 58.
Riens B & Heldt HW, Plant Physiol, 98 (1992) 573.
Huber SC, Huber JL, Campbell WH & Redinbaugh MG, Plant Physiol, 100 (1992) 706.
Kaiser WM & Spill D, Plant Physiol, 96 (1991) 368.
Jaworski EG, Biochem Biophys Res Commun, 43 (1971) 1274.
Sawhney SK, Naik MS & Nicholas DJD, Biochem Biophys Res Commun, 81 (1978) 1209.
Hageman RH & Hucklesby DP, Methods Enzymol, 23 (1971) 491.
Sawhney SK, Naik MS & Nicholas DJD, Nature, 272 (1978) 647.
Reed AJ, Canvin DT, Sherrard JH & Hageman RH, Plant Physiol, 71 (1983) 291.
Voneyama T, Plant Cell Physiol, 22 (1981) 1507.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Salalkar, B.K., Shaikh, R.S., Naik, R.M. et al. Changes in Leaf Nitrate Reductase Activity In Vivo and In Vitro During Light-Dark Transitions. J. Plant Biochem. Biotechnol. 8, 37–40 (1999). https://doi.org/10.1007/BF03263055
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263055