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Transforming Growth Factor β-1 −509C>T Polymorphism in Indian Patients with Primary Open Angle Glaucoma

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Abstract

Background: Extracellular matrix (ECM) accumulation in the trabecular meshwork tissues of glaucoma patients has been demonstrated as one of the factors that contribute to glaucoma pathology. Transforming growth factor-β (TGFβ) has its fundamental function in regulating the ECM molecules and has been implicated in glaucoma pathology. In this study, the association of the TGFB1 −-509C>T single nucleotide polymorphism (SNP) with primary open angle glaucoma (POAG) in patients from India is analyzed.

Methods: One-hundred and six POAG patients and 104 controls were selected after comprehensive ophthalmic examinations. TGFB1 alleles were typed by restriction enzyme digestion with the isoschizomer Eco81I of Bsu36I, whose site is altered by the −509C>T SNP, and statistically analyzed for any significant association. Two clinical variables, vertical cup disc ratio (CDR) and intraocular pressure (IOP), were compared at diagnosis by the Mann-Whitney test for any significant association with the polymorphism.

Results: Statistical analysis between the two groups did not suggest any significant difference in the distribution of allele and genotype frequencies. The Mann-Whitney test did not show any significant p value for the clinical parameters IOP (p = 0.29 and 0.59) and CDR (p = 0.26 and 0.17).

Conclusions: The current study shows that the TGFB1 ∼-509C>T polymorphism might not be associated with POAG. Analysis of the other polymorphisms in the regulatory region of the TGFB1 gene could give a better understanding of the role of TGFβ in POAG pathogenesis.

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References

  1. Yue BY. The extracellular matrix and its modulation in the trabecular meshwork. Surv Ophthalmol 1996; 40: 379–90

    Article  PubMed  CAS  Google Scholar 

  2. Lip PL, Felmeden DC, Blann AD, et al. Plasma vascular endothelial growth factor, soluble VEGF receptor FLT-1, and von Willebrand factor in glaucoma. Br J Ophthalmol 2002; 86: 1299–302

    Article  PubMed  CAS  Google Scholar 

  3. Ochiai Y, Ochiai H. Higher concentration of transforming growth factor-beta in aqueous humor of glaucomatous eyes and diabetic eyes. Jpn J Ophthalmol 2002; 46: 249–53

    Article  PubMed  CAS  Google Scholar 

  4. Hu DN, Ritch R. Hepatocyte growth factor is increased in the aqueous humor of glaucomatous eyes. J Glaucoma 2001; 10: 152–7

    Article  PubMed  CAS  Google Scholar 

  5. Signaling pathway database [online]. Available from URL: http://www.grt.kyushu-u.ac.jp/spad/account/ligand/tgf-beta.html [Accessed 2007 Mar 12]

  6. Pasquale LR, Dorman-Pease ME, Lutty GA, et al. Immunolocalization of TGF-beta 1, TGF-beta 2, and TGF-beta 3 in the anterior segment of the human eye. Invest Ophthalmol Vis Sci 1993; 34: 23–30

    PubMed  CAS  Google Scholar 

  7. Pena JD, Taylor AW, Ricard CS, et al. Transforming growth factor beta isoforms in human optic nerve heads. Br J Ophthalmol 1999; 83: 132–3

    Article  Google Scholar 

  8. Fukuchi T, Ueda J, Hanyu T, et al. Distribution and expression of transforming growth factor-beta and platelet-derived growth factor in the normal and glaucomatous monkey optic nerve heads. Jpn J Ophthalmol 2001; 45: 592–9

    Article  PubMed  CAS  Google Scholar 

  9. Li J, Tripathi BJ, Ramesh C. Tripathi1 modulation of pre-mRNA splicing and protein production of fibronectin by TGF-b2 in porcine trabecular cells pathway. Invest Ophthalmol Vis Sci 2000; 41: 3437–43

    PubMed  CAS  Google Scholar 

  10. Kirwan RP, Crean JK, Fenerty CH, et al. Effect of cyclical mechanical stretch and exogenous transforming growth factor-beta1 on matrix metalloproteinase-2 activity in lamina cribrosa cells from the human optic nerve head. J Glaucoma 2004; 3: 327–34

    Article  Google Scholar 

  11. Tamm ER, Russell P, Epstein LD, et al. Modulation of myocilin/TIGR expression in human trabecular meshwork. Invest Ophthalmol Vis Sci 1999; 40: 2577–82

    PubMed  CAS  Google Scholar 

  12. Grainger DJ, Heathcote K, Chiano M, et al. Genetic control of the circulating concentration of transforming growth factor type betal. Hum Mol Genet 1999; 8: 93–7

    Article  PubMed  CAS  Google Scholar 

  13. Awad MR, El-Gamel A, Hasleton P, et al. Genotypic variation in the transforming growth factor-beta1 gene: association with transforming growth factor-beta1 production, fibrotic lung disease, and graft fibrosis after lung transplantation. Transplantation 1998; 66: 1014–20

    Article  PubMed  CAS  Google Scholar 

  14. Arvind H, Paul PG, Raju P, et al. Methods and design of the Chennai Glaucoma Study. Ophthalmic Epidemiol 2003; 10: 337–48

    Article  PubMed  Google Scholar 

  15. REBASE® [online]. Available from URL: http://rebase.neb.com/rebase/rebase.html [Accessed 2007 Mar 12]

  16. Flugel-Koch C, Ohlmann A, Piatigorsky J, et al. Disruption of anterior segment development by TGF-beta1 over expression in the eyes of transgenic mice. Dev Dyn 2002; 225: 111–25

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors would like to acknowledge the Chennai Willingdon Corporate Foundation, Chennai, India.

The authors have no conflicts of interest that are directly relevant to the content of this study.

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Authors and Affiliations

  1. SNONGC Department of Genetics and Molecular Biology, Vision Research Foundation, 18 College Road, Nungambakkam, Sankara Nethralaya, Chennai, 600006, Tamil Nadu, India

    Sarangapani Sripriya, Tirumalai Karthiyayini &  Govindasamy Kumaramanickavel

  2. Department of Glaucoma, Medical Research Foundation, Sankara Nethralaya, Chennai, India

    Ronnie George, Hemamalini Arvind, Mani Baskaran, Prema Raju, S. V. Ramesh & Lingam Vijaya

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  1. Sarangapani Sripriya
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  2. Ronnie George
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  3. Hemamalini Arvind
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  4. Mani Baskaran
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  5. Prema Raju
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  6. S. V. Ramesh
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  7. Tirumalai Karthiyayini
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  8. Lingam Vijaya
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  9. Govindasamy Kumaramanickavel
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Sripriya, S., George, R., Arvind, H. et al. Transforming Growth Factor β-1 −509C>T Polymorphism in Indian Patients with Primary Open Angle Glaucoma. Mol Diag Ther 11, 151–154 (2007). https://doi.org/10.1007/BF03256236

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  • DOI: https://doi.org/10.1007/BF03256236

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