Summary
We compared the ability of two different diets containing 6 per cent of maize oil and 6 per cent offish oil to modify: firstly the enzyme induction by phenobarbital and secondly the phenobarbital hydroxylation by the liver either in vivo or during in vitro perfusions. The presence of fish oil in the diet increased the cyt P 450 content and the bilirubin glucuronosyl transferase activity. The two induction effects promoted by the association of the phenobarbital treatment and the eating of the fish oil were not additive and it was found that the phenobarbital induction effect was decreased by the fish oil consumption. Phenobarbital and p-hydroxyphenobarbital kinetics were different in the two groups of animals. Phenobarbital was more slowly eluted in the fish oil fed than in the maize oil fed rats while p-hydroxyphenobarbital was more slowly eluted by the fish oil-fed rat livers.
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References
Norred W.P., Wade A.E., (1972), Dietary fatty-acids induced alterations of hepatic microsomal drugs metabolism. Biochem. Pharmacol., 21: 2887–2897.
Mounié J., Goudonnet H., Dumas M., Escousse A., Truchot R., (1981), Facteurs nutritionnels de 1 induction enzy matique chez le Rat: influence de la composition quantitative et qualitative lipidique. J. Pharmacol., 12: 3–14.
Mounié J., Bereksi-Reguig K., Goudonnet H., Escousse A., Truchot R., (1983), Liver microsome phospholipids and Cyt. P 450 concentration in PB treated rats feds on differents diets. Life Sci., 32: 503–510.
Mounié J., Faye B., Magdalou J., Goudonnet H., Truchot R., (1986), Modulation of UDPGT-glucuronosyltransferase activity in Rats by dietary lipids. J. Nutr., 116: 2034–2043.
Gaillard D., Chamoiseau G., Suschetet M., (1976), Effet de 1 acide tannique et du métabisulfite de potassium en association avec léthanol, sur quelques activités de détoxication enzymatique des microsomes hépatiques du rat., Fed. Cosmet. Toxicol., 14: 123–132.
Magdalou J., Steinmetz D., Batt A.M., Poullain B., Siest G., Debry G., (1979), The effect of dietary sulfur containing aminoacids on the activity of drug metabolizing enzymes in Rat liver microsomes., J. Nutr., 109: 864–871.
Tien M;, Aust S.D., (1982), Rabbit liver microsomal lipid peroxydation. The effect of lipid on the rate of peroxydation. Biochem. Biophys. Act., 712: 1–9.
Omura T., Sato R., (1964), The carbon monoxyde pigment of liver microsomes. Evidence of its hemoprotein nature. J. Biol. Chem., 239: 2370–2373.
Kergueris M.F., Larousse C., Guillerme G., (1982), Induction enzymatique chez l’ animal. Mesure de l’ activité de quelques enzymes des microsomes hépatiques. J. Pharmacol., 13: 585–591.
Heirwegh K.P.M., Van de Vijver M., Fevery J., (1972), Assay and properties of digitonin-activated Bilirubin UDPGT from rat liver. Biochem. J., 129: 605–618.
Mounié J., Champion L., Goudonnet H., Escousse A., Truchot R.C., (1988), Inductive effects of fenofibrate on phenobarbital metabolism. Fund. Clin. Pharm., 2: 259–265.
Dumas M., Hellion P., Escousse A., (1981), Technique de perfusion de foie perfus isol. J. Pharmacol., 2: 211–217,.
Wallis E.D., (1971), Effect of lipid peroxydation on membrane bound enzyme of the endoplasmic reticulum. Biochem. J., 123: 983–991.
Hammer C.T., Wills E.D., (1979), The effect of dietary fats on the composition of the liver endoplasmic reticulum and oxydative drug metabolism. Brit. J. Nut., 41: 465–475.
Gower J.D., Sayer J.W. and Wills E.D., (1986), The effects of dietary lipids and antioxydants on the activity of epoxide hydrolase in the rat liver and intestine. Biochem. Pharmacol., 35: 1965–1969.
Savolainen M.J., Arranlo A.J., Hassinen P.V., Luoma P.V., Olavi Pelkonen R., Sotaieni E.A., (1985), Relationship between lipid composition and drug metabolizing capacity of human liver. Eur. J. Clin. Pharmacol., 27, 727–732.
Lambert L., Wills E.D., (1977), The effect of dietary lipids peroxydes, sterols and oxydized sterols on cyt. P 450 and oxydative demethylation in the E.R. Biochem. Pharmacol., 26: 1417–1421.
Oliw E.H., Guenguerich F.P., Oates J.A., (1982), Oxygenation of arachidonic acid by mono- oxygenases: isolation and metabolism of four epoxydes intermediates. J. Biol. Chem., 257: 3771–3781.
Chacos N., Falk J.R., Wixtrom C., Capdevila J., (1982), Novel epoxydes formed during the liver cytochrome P 450 oxydation of arachidonic acid. Biochem. Biophys. Res. Commun., 104: 916–922.
Wills E.D., (1969), Lipid peroxyde formation in microsomes. Relationship of hydroxylation to lipid peroxyde formation. Biochem. J., 113: 333–341.
Bartosek I., Guatani A., Garatlini S., Somozzani I., (1974), Microsomal enzyme activity in perfused Rat liver. Biochem. Pharmacol., 23: 599–605.
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Mounie, J., Goudonnet, H., Prevost, C. et al. Nutritional factors of microsomal enzymatic induction: influence of lipidic components of the diet. Eur. J. Drug Metab. Pharmacokinet. 15, 265–271 (1990). https://doi.org/10.1007/BF03190214
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DOI: https://doi.org/10.1007/BF03190214