Abstract
Previous study showed that CCl4 administration evoked a rapid decrease in cytochrome P450 2E1 protein soon after the exposure due to posttranslational inhibition(Biochem. Biophys. Res. Commun. 179:449–454, 1991). In this report, aniline hydroxylase and the amounts of immunoreactive P450 2E1 were rapidly decreased during day 1 to 2 and recovered during day 3 to 4 after a single dose of CCl4. The activity of pentoxyresorufin-0-dealkylase was also suppressed at day 1 and began to repair from day 2. However, the decrease in immunoreactive P450 2C content was not observed. The decreases in P450 2E1 enzyme activity and immunoreactive protein by acute CCl4 treatment were accompanied by a decline in P450 2E1 mRNA level. The data thus suggested a pretranslational reduction of P450 2E1 during day 1 to 2 after acute CCl4 treatment.
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Park, S.M., Park, E.J., Ko, G. et al. A study for regulation of ethanol-inducible P450(CYP2E1) on CCl4-induced hepatic damage. Arch. Pharm. Res. 18, 179–182 (1995). https://doi.org/10.1007/BF02979192
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DOI: https://doi.org/10.1007/BF02979192