Abstract
Polymerase Chain Reaction (PCR) technique were conducted to identify the phytoplasma disease of sugarcane in two different locations of the sugarcane plantations namely Sevanagala and Uda Walawe in Sri Lanka. The results revealed that the samples of diseased plants of the two plantations give the same Restriction Fragment Length Polymorphism (RFLP) pattern with both restriction enzymes (Alul and Rsal) similar to Sugarcane White Leaf Disease (WLD). This is the first authentic confirmation of occurrence of WLD in Sri Lanka.
Similar content being viewed by others
References
Corbett M.K., Misra, S.R. and Singh, Kishan (1972). Grassy shoot disease of sugarcane IV Association of mycoplasma like bodies.Plant Science 3: 80–82.
Cronje, C.P.R., Tymon, A.M., Jones, P. and Bailey, R.A. (1998). Association of a phytoplasma with a yellow leaf syndrome of sugarcane in Africa.Annals of Applied Biology,133: 177–186.
Deng, S. and Hiruki, C. (1991). Amplification of 16S rRNA genes from culturable and non culturable molicutes.Journal of Microbiological Methods,14: 53–61.
Gundersen, D.E. and Lee, I.M. (1996). Ultrasensitive detection of phytoplasmas by nested PCR using two universal primer pairs.Phytopathologia Mediterranea,35: 144–151.
Kabir, A.K.M., Tsuno, K., Mian, I.H. and Rahman, S. (1988). Mycoplasma like organism in Grassy Shoot Disease of Sugarcane.Bangladesh Journal of Sugarcane,10: 1–4.
Liang, K.C. (1962). White leaf disease of sugarcane.Taiwan Sugar,9: 1–5.
Marcone, C., Ragozzino, A. and Seemuller, E. (2001). Sugarcane white leaf and sugarcane grassy shoot disease: biology and taxonomic characterization of the associated phytoplasmas. pp. 83–98.In : Sugarcane Pathology, Vol. II. Virus and phytoplasma disease, (eds) G.P. Rao, R.E. Ford, M. Tosic and D.D. Teakle), Science Publishers, Inc., Enfield, USA.
Nakashima, K. and Murata, N. (1993). Destructive plant diseases caused by micoplasma-like organisms in Asia.Outlook on Agriculture 22: (1) 53–58.
Raj Bhansali, R. and Shukla, U.S. (1985). Stains for detection mycoplasma like organisms and anatomical changes in grassy shoot diseased sugarcane leaves.Journal of Plant Anatomy and Morphology,2 (1): 55–61.
Smart, CD., Schneider, B., Blomquist, D.I., Guerra, I.J., Harison, N.A., Ahrens, U., Lorenz, K.H., Seemuller, E. and Kirkpalrick, B. (1996). Phytoplasma-specific PCR primers based on sequences of the 16S-23S rRNA spacer region.Applied and Environmental Microbiology,62: 2988–2993.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kumarasinghe, N.C., Jones, P. Identification of white leaf disease of sugarcane in Sri Lanka. Sugar Tech 3, 55–58 (2001). https://doi.org/10.1007/BF02945532
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02945532