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Aptamers as functional nucleic acids:In vitro selection and biotechnological applications

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Abstract

Aptamers are functional nucleic acids that can specially bind to proteins, peptides, amino acids, nucleotides, drugs, vitamins and other organic and inorganic compounds. The aptamers are identified from random DNA or RNA libraries by a SELEX (systematic evolution of ligands by exponential amplification) process. As aptamers have the advantage, and potential ability to be released from the limitations of antibodies, they are attractive to a wide range of therapeutic and diagnostic applications. Aptamers, with a high-affinity and specificity, could fulfil molecular the recognition needs of various fields in biotechnology. In this work, we reviewed some aptamer selection techniques, properties, medical applications of their molecules and their biotechnological applications, such as ELONA (enzyme linked oligonucleotide assay), flow cytometry, biosensors, electrophoresis, chromatography and microarrays.

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  1. Division of Molecular and Life Sciences, Pohang University of Science and Technology, 790-784, Pohang, Korea

    Kyung Man You, Sang Hyun Lee, Aesul Im & Sun Bok Lee

  2. Department of Chemical Engineering, Pohang University of Science and Technology, 790-784, Pohang, Korea

    Sang Hyun Lee, Aesul Im & Sun Bok Lee

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You, K.M., Lee, S.H., Im, A. et al. Aptamers as functional nucleic acids:In vitro selection and biotechnological applications. Biotechnol Bioproc E 8, 64–75 (2003). https://doi.org/10.1007/BF02940259

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