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A microculture containing TH2 and dendritic cells supports the production of IgA by clones from both primary and IgA memory B cells and by single germinal center B cells from Peyer's patches

  • B Cell Differentiation
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References

  1. Gearhart PJ, Cebra JJ: Differentiated B lymphocytes. Potential to express particular antibody variable and constant region depends on site of lymphoid tissue and antigen load. J Exp Med 1979;149:216–227.

    Article  PubMed  CAS  Google Scholar 

  2. Fuhrman JA, Cebra JJ: Special features of the priming process for a secretory IgA response, B cell priming with cholera toxin. J Exp Med 1981; 153:534–544.

    Article  PubMed  CAS  Google Scholar 

  3. Lebman DA, Griffin PM, Cebra JJ: Relationship between expression of IgA by Peyer's patch cells and functional IgA memory cells. J Exp Med 1987;166:1405–1418.

    Article  PubMed  CAS  Google Scholar 

  4. Schrader CE, George A, Kerlin RL, Cebra JJ: Dendritic cells support production of IgA and other non-IgM isotypes in clonal microculture. Int Immunol 1990;2:563–570.

    Article  PubMed  CAS  Google Scholar 

  5. Kerlin RL, Cebra JJ, Weinstein PD, Prendergast RA: Sea Star Factor blocks development of T dependent antibody secreting clones by preventing lymphokine secretion. Cell Immunol, in press.

  6. Kotloff DB, Cebra JJ: Effect of TH-lines and clones on the growth and differentiation of B cell clones in microculture. Mol Immunol 1988;25: 147–155.

    Article  PubMed  CAS  Google Scholar 

  7. Steinman RM, Kaplan G, Witmer MD, Cohn ZA: Identification of a novel cell type in peripheral lymphoid organs of mice. V. Purification of spleen dendritic cells, new surface markers, and maintenance in vitro. J Exp Med 1979;149:1–16.

    Article  PubMed  CAS  Google Scholar 

  8. Spalding DM, Koopman WJ, Eldridge JH, McGhee JR, Steinman RM: Accessory cells in murine Peyer's patch. I. Identification and enrichment of a functional dendritic cell. J Exp Med 1983;157:1646–1659.

    Article  PubMed  CAS  Google Scholar 

  9. Inaba K, Witmer MD, Steinman RM: Clustering of dendritic cells, helper T lymphocytes, and histocompatible B cells during primary antibody responses in vitro. J Exp Med 1984;160:858–876.

    Article  PubMed  CAS  Google Scholar 

  10. Spalding DM, Griffin JA: Different pathways of differentiation of pre-B cell lines are induced by dendritic cells and T cells from different lymphoid tissues. Cell 1986;44:507–515.

    Article  PubMed  CAS  Google Scholar 

  11. Hebbard GS, Pike BL, Nossal GJV: Single cell studies on hapten-specific B cells: Response to T cell-dependent antigens. Proc Natl Acad Sci USA 1984;81:2479–2483.

    Article  PubMed  CAS  Google Scholar 

  12. Monroe JG, Blank KJ, Owen CS, Sykes N: Signalling through sIgA on a novel B lymphoma. Mol Immunol 1989;26:593–599.

    Article  PubMed  CAS  Google Scholar 

  13. Nieuwenhuis P, Opstelten D: Functional anatomy of germinal centers. Am J Anat 1984;170:421–435.

    Article  PubMed  CAS  Google Scholar 

  14. Reichert RA, Gallatin WM, Weissman IL, Butcher EC: Germinal center B cells lack homing receptors necessary for normal lymphocyte recirculation. J Exp Med 1983;157:813–827.

    Article  PubMed  CAS  Google Scholar 

  15. George A, Cebra JJ: Responses of single germinal center B cells in T-dependent microculture. Proc Natl Acad Sci USA 1991;88:11–15.

    Article  PubMed  CAS  Google Scholar 

  16. Bishop GA, Haughton G: Rote of the interleukin-2 receptor in differentiation of a clone of Ly-1+ B cells. J Immunol 1987;138:3308–3313.

    PubMed  CAS  Google Scholar 

  17. Ikegami S, Taguchi T, Ohashi M: Aphidicholin prevents mitotic cell division by interfering with the activity of DNA polymerase-α. Nature (London) 1978;275:458–459.

    Article  CAS  Google Scholar 

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Cebra, J.J., George, A. & Schrader, C.E. A microculture containing TH2 and dendritic cells supports the production of IgA by clones from both primary and IgA memory B cells and by single germinal center B cells from Peyer's patches. Immunol Res 10, 389–392 (1991). https://doi.org/10.1007/BF02919727

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