Abstract
Hairy roots ofBrugmansia candida were used to bioconvert hydroquinone into arbutin. The highest bioconversion, with the lowest damage to the cells, was attained when concentrations of 20–40 mg/L hydroquinone were used. Sugars (sucrose, glucose, mannitol, and sorbitol) at concentrations of 30–120 g/L enhanced bioconversion, and, of these, sucrose was the most effective. Two different free-radical scavengers were also tested: sodium benzoate and gallic acid. The first one diminished biotransformation efficiency; gallic acid did not affect biotransformation at all. Preliminary permeabilization treatments tested failed to liberate arbutin into the medium, and provoked a total loss in cell viability.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Reynolds, J. E. F. (1989), in theExtra Pharmacopoeia, 29th ed., Martindale, ed., Pharmaceutical, pp. 922.
Akiu, S., Suzuki, Y., Fujinuma, Y., Asahara, T., and Fukuda, M. (1988),Proc. Jpn. Soc. Invest. Dermatol. 12, 138–139.
Itabashi, M., Aihara, H., Inoue, T., Yamate, J., Sanni, S., Tajima, M., Tanaka, C., and Wakisaka, Y. (1988),Iyakuhin Kenkyu 19, 282–297.
Suga, T. and Hirata, T. (1990),Phytochemistry 29, 2393–2406.
Tabata, M., Ikeda, F., Hiraoka, N., and Konoshima, M. (1976),Phytochemistry 15, 1225–1229.
Tabata, M., Umetani, Y., Ooya, M., and Tanaka, S. (1988),Phytochemistry 27, 809–813.
Scholten, H. J., Schans, M. J., and Somhorst, I. P. M. (1991),Plant Cell. Tissue Organ Cult. 26, 173–178.
Flores, H. E. and Curtis, W. R. (1992),Ann. NY Acad. Sci. 665, 188–209.
Flores, H. E., Hoy, M. W., and Pickard, J. J. (1987),Trends Biotechnol. 5, 64–69.
Flores, H. E. (1992),Chem. Ind. 10, 374–377.
Flores, H. E., Dai, Y., Freyer, A. J., and Michaels, P. J. (1994),Plant Physiol. Biochem. 32, 511–519.
Pitta-Alvarez, S. I. and Giulietti, A. M. (1995),In Vitro Cell. Dev. Biol. Plant. 31, 215–220.
Gamborg, O. L., Miller, R. A., and Ojima, K. (1968),Exp. Cell. Res. 50, 148–151.
Meravy, L. (1987),Biol. Plantarum 29, 247–252.
Lutterbach, R. and Stockigt, J. (1994),Helvetica Chimica Acta 77, 2153–2161.
Larsen, W., Hsu, J. T., Flores, H. E., and Humphrey, A. E. (1993),Biotechnol. Techniques 7, 557–562.
Brodelius, P. and Nilsson, K. (1983),Eur. J. Appl. Microbiol. Biotechnol. 17, 275–280.
Duskova, J., Jahodar, L. and Dusek, J. (1990),Ceskoslovenska Farmacie 39, 452–455.
Barz, W. and Koster, J. (1981), inThe Biochemistry of Plants, vol. 7, Stumpe, P. K. and Conn, E. E., eds., Academic, New York, pp. 35–84.
Yokohama, M., Inomata, S., Seto, S. and Yanagi, M. (1990),Plant Cell Physiol. 31, 551–555.
Suga, T. and Hirata, T. (1990),Phytochemistry 29, 2393–2406.
Dornenburg, H. and Knorr, D. (1995),Enzyme Microbiol Technol. 17, 674–684.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Casas, D.A., Pitta-Alvarez, S.I. & Giulietti, A.M. Biotransformation of hydroquinone by hairy roots ofBrugmansia candida and effect of sugars and free-radical scavengers. Appl Biochem Biotechnol 69, 127–136 (1998). https://doi.org/10.1007/BF02919394
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02919394