Summary
Amplification of genomic DNA encoding oncogenes such as HER-2 (syn. c-erbB2/c-neu) may be substantially involved in the initiation and progression of breast cancer. In order to refine and facilitate the quantitative analysis of HER-2 amplification in breast cancer, differential polymerase chain reaction (PCR) was performed on DNA derived from single cryosections of tumor tissue. This technique is based on the simultaneous amplification of a potentially amplified oncogene (HER-2) and a reference gene (IFN-gamma). Differential PCR yielded reproducible results that were in agreement with gene copy quantification using the dot blot technique. Thus we suggest differential PCR to be a reliable and rapid method for determining relative gene dosage in a minute amount of tumour tissue.
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References
Ali IU, Campbell G, Lidereau R, Callahan R (1988) Amplification of c-erbB2 and aggressive breast tumors? Science 240:1795–1796
Allred DC, Clark GM, Molina R, Tandon AK, Schnitt SJ, Gilchrist KW, Osborne CK, Tormey DC, McGuire WL (1992) Overexpression of HER-2/neu and its relationship with other prognostic factors change during progression of in situ to invasive breast cancer. Hum Pathol 23:974–979
Anderson TJ (1992) c-erbB-2 oncogene in breast cancer: the right target or a decoy? Editorial, Hum Pathol 23:971–973
Ciocca DR, Fujimura FK, Tandon AK, Clark GM, Mark C, Lee Chen G-J, Pounds GW, Vendely P, Owens MA, Pandian MR, McGuire WL (1992) Correlation of HER-2/neu amplification with expression and with other prognostic factors in 1103 breast cancers. JNCI 84:1272–1282
Crabbe DCG, Peters J, Seeger RC (1992) Rapid detection of mycn gene amplification in neuroblastomas using polymerase chain reaction. Diagn Mol Pathol, Vol 1(4):229–234
Feinberg AP, Vogelstein B (1983) A technique for labelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Frye RA, Benz CC, Liu E (1989) Detection of amplified oncogenes by differential polymerase chain reaction. Oncogene 4:1153–1157
Liu E, Thor A, He M, Barcos B, Ljung B-M, Benz C (1992) The HER-2 (c-erbB-2) oncogene is frequently amplified inin situ carcinomas of the breast. Oncogene 7:1027–1032
Neubauer A, Neubauer B, He M, Effert P, Iglehart JD, Frye RA, Liu E (1992) Analysis of gene amplification in archival tissue by differential polymerase chain reaction. Oncogene 7:1019–1023
Slamon DJ, Clark GM, Wong SG, Levin WJ, Ullrich A, McGuire WL (1987) Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 235:177–182
Tsuda H, Hirohashi S, Shimosato Y, Hiroti T, Tsugane S, Yamamoto H, Miyajima N, Toyoshima K, Yamamoto T, Yokota J, Yoshida T, Sakamoto H, Terada M, Sugimura T (1989) Correlation between long-term survival in breast cancer patients and amplification of two putative oncogene-coamplification units: hst1/int2 and c-erbB2/ear-1. Cancer Res 49:3104–3108
van der Vijver MJ, Peterse JL, Mooi WJ, Wisman P, Nusse R (1988)NEU-Protein Overexpression in breast cancer. N Engl J Med 319:1239–1245
Venter DJ, Tuzi NL, Kumar S, Gulick WJ (1987) Overexpression of the c-erbB2 oncoprotein in human breast cancer: immunohistological assessment correlates with gene amplification. Lancet 2:69–71
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Friedrichs, K., Lohmann, D. & Höfler, H. Detection of HER-2 oncogene amplification in breast cancer by differential polymerase chain reaction from single cryosections. Virchows Archiv B Cell Pathol 64, 209–212 (1993). https://doi.org/10.1007/BF02915114
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DOI: https://doi.org/10.1007/BF02915114