Abstract
The basis of low seed set was investigated in a diploid hybrid population with germplasm from the cultivated speciesSolanum tuberosum spp.tuberosum andS. tuberosum ssp.phureja and the wild speciesS. chacoense. Controlled crosses were performed following an incomplete diallel mating design which included the hybrid population and the parental species. Pollen grain germination and pollen tube growth were observed with fluorescence microscopy in 174 intrapopulation and interspecific combinations of genotypes. Fifty percent of the combinations of genotypes within the hybrid population and 46% of those between this population and the parental species were incompatible; overall, 71% of the interspecific combinations of genotypes were compatible when plants of the hybrid population were used as females, vs. 5% when used as males. Although the site of reaction could vary in a given intrapopulation or interspecific cross with the combinations of genotypes, pollen tube inhibition occurred in the first third of the style in 42% of them. Since the gene pool of the hybrid population had been widened with germplasm from other geographic areas and the genotypes ofS. tuberosum ssp.tuberosum, S. chacoense andS. tuberosum ssp.phureja used in this study were not the ones involved in the original crosses, it is concluded that the S-locus is not controlling the incompatibility reaction but rather that a cross-incompatibility system, possibly governed by more than one locus, is acting.
Resumen
Se investigó la base de la producción baja de semilla en una población híbrida diploide con germoplasma de las especies cultivadasSolanum tuberosum ssp.tuberosum yS. tuberosum ssp.phureja y la especie silvestreS. chacoense. Los cruzamientos controlados se realizaron siguiendo un diseño dialélico incomplete, el cual incluyo la población híbrida y las especies parentales. Se observa la germinacién del grano de polen y el crecimiento del tubo de polen con un microscopio de fluorescencia en 174 combinaciones intrapoblacionales interespecificas de genotipos. El 50% de las combinaciones de genotipos en la población híbrida y el 46% de aquéllas entre esta población y las especies parentales fueron incompatibles; en total, el 71% de las combinaciones interespecíficas de genotipos fueron compatibles cuando las plantas de la población híbrida se usaron como hembras vs. el 5% cuando se usaron como machos. Aunque el lugar de reacción podría variar en en cruzamiento intrapoblaciónale interespecifico dada con las combinaciones de genotipos, la inhibición del tubo de polen se dio en el primer tercio del estilo en el 42% de ellas. Desde que el acervo de genes/de la población híbrida se amplió con germoplasma de otras areas geográficas y los genotipos deS. tuberosum ssp.tuberosum, S. chacoense y S. tuberosum ssp.phureja empleados en este estudio no fueron los involucrados en los cruzamientos originales, se concluye que el locus S no controla la reacción de incompatibilidad sino que esta un sistema de incompatibilidad cruzada, posiblemente controlado por mas de un locus.
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Abbreviations according to Simmonds (1963).
Endosperm Balance Numbers (Johnstonet al., 1980).
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Camadro, E.L., Verde, L.A. & Marcellán, O.N. Pollen-pistil incompatibility in a diploid hybrid potato population with cultivated and wild germplasm. Am. J. Pot Res 75, 81–85 (1998). https://doi.org/10.1007/BF02883881
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DOI: https://doi.org/10.1007/BF02883881