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Application of electrospun silk fibroin nanofibers as an immobilization support of enzyme

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Abstract

Silk fibroin (SF) nanofibers were prepared by electrospinning and their application as an enzyme immobilization support was attempted. By varying the concentration of SF dope solution the diameter of SF nanofiber was controlled. The SF nanofiber web had high capacity of enzyme loading, which reached to 5.6 wt%. The activity of immobilizedα-chymotrypsin (CT) on SF nanofiber was 8 times higher than that on silk fiber and it increased as the fiber diameter decreased. Sample SF8 (ca. 205 nm fiber diameter) has excellent stability at 25°C by retaining more than 90 % of initial activity after 24 hours, while sample SF11 (ca. 320 nm fiber diameter) shows higher stability in ethanol, retaining more than 45% of initial activity. The formation of multipoint attachment between enzyme and support might increase the stability of enzyme. From these results, it is expected that the electrospun SF nanofibers can be used as an excellent support for enzyme immobilization.

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Authors and Affiliations

  1. Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, 151-921, Seoul, Korea

    Ki Hoon Lee, Chang Seok Ki, Doo Hyun Baek, Gyung Don Kang, Dae-Woo Ihm & Young Hwan Park

  2. Department of Innovative Industrial Technology, Hoseo University, 336-795, Asan, Korea

    Dae-Woo Ihm

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  1. Ki Hoon Lee
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  2. Chang Seok Ki
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  3. Doo Hyun Baek
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  4. Gyung Don Kang
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  5. Dae-Woo Ihm
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  6. Young Hwan Park
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Correspondence to Young Hwan Park.

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Lee, K.H., Ki, C.S., Baek, D.H. et al. Application of electrospun silk fibroin nanofibers as an immobilization support of enzyme. Fibers Polym 6, 181–185 (2005). https://doi.org/10.1007/BF02875641

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  • DOI: https://doi.org/10.1007/BF02875641

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