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The restriction-modification system inStreptomyces flavopersicus

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Abstract

To clone bifunctional vectors in streptomycetes, it was necessary to define the restriction-modification system ofStreptomyces flavopersicus. Plasmid DNA from bifunctional vectors pIJ699 and pXED3-13, isolated fromE. coli strains with different methylation systems:E. coli DH5α (dam + dcm +),E. coli MB5386(dam dcm), E. coli CB51 (dam dcm +),E. coli NM544 (dam + dcm), was used for transformation of protoplasts from strainS. flavopersicus. Restriction ofdcm-methylated DNA fromS. flavopersicus was established. As a host in the intermediate cloning strainE. coli NM544 (dam + dcm) should be used, as thedcm-transmethylase-dependent strainS. flavopersicus does not process DNA from this strain.

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Authors and Affiliations

  1. Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    D. Lyutzkanova, M. Stoilova-Disheva & V. Peltekova

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  1. D. Lyutzkanova
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  2. M. Stoilova-Disheva
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  3. V. Peltekova
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Correspondence to D. Lyutzkanova.

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Lyutzkanova, D., Stoilova-Disheva, M. & Peltekova, V. The restriction-modification system inStreptomyces flavopersicus . Folia Microbiol 46, 119–122 (2001). https://doi.org/10.1007/BF02873588

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