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HPLC analysis of methylated amino acids: Methylated amino acids on HPLC

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Abstract

Various naturally occuring, methylated amino acid derivatives were resolved on high performance liquid chromatography (HPLC), usingo-phthaldialdehyde as a fluorogenic reagent.

We separated ε-N-monomethyllysine, ε-N-dimethyllysine, and ε-N-acetyllysine from lysine derivatives. NG-Monomethylarginine and NG-dimethylarginine were separated from arginine derivatives. However, ε-N-monomethyllysine and ε-N-trimethyllysine, NG, NG-dimethylarginine and NG, NG-dimethylarginine were not resolved under the conditions employed. S-Methylmethionine, S-methylcysteine, and 1-N-methylhistidine or 3-N-methylhistidine were clearly separated from their reference amino acids, even though 1-N-methyl- and 3-N-methylhistidine could not be separated.

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Authors and Affiliations

  1. Fels Research Institute, Temple University School of Medicine, 19140, Philadelphia, PA, U.S.A.

    Kwang Sook Park, Sangduk Kim & Woon Ki Paik

  2. Department of Biochemistry, School of Pharmacy, Sung Kyun Kwan University, Kyunggi-Do, 170, Suwon, Korea

    Sung-Youl Hong & Hyang Woo Lee

Authors
  1. Kwang Sook Park
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  2. Sung-Youl Hong
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  3. Hyang Woo Lee
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  4. Sangduk Kim
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  5. Woon Ki Paik
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Park, K.S., Hong, SY., Lee, H.W. et al. HPLC analysis of methylated amino acids: Methylated amino acids on HPLC. Arch. Pharm. Res. 9, 15–18 (1986). https://doi.org/10.1007/BF02857700

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  • DOI: https://doi.org/10.1007/BF02857700

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