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The influence of contact guidance on the orientation of colonies of subcultured vascular smooth muscle cells

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Summary

Subcultures of smooth muscle cells derived from rat thoracic aorta were grown on plane plastic substrata and on plastic substrata having ridges molded in them by a heated, ruled template. The cells were found to have a very high degree of contact guidance when distributed sparsely on the ridged substrata. When the cell density increased multilayered, elongated colonies formed. On plane substrata these were irregular, curved, and disposed in all directions. On the ridged substrata, however, the colonies were straight, evenly spaced, and positioned at right angles to the ridges.

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Authors and Affiliations

  1. Department of Anatomy, Health Sciences Centre, The University of Western Ontario, N6A 5C1, London, Ontario, Canada

    Robert C. Buck

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  1. Robert C. Buck
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Supported by Grant MT1011 from the Medical Research Council of Canada.

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Buck, R.C. The influence of contact guidance on the orientation of colonies of subcultured vascular smooth muscle cells. In Vitro 18, 783–788 (1982). https://doi.org/10.1007/BF02796502

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  • DOI: https://doi.org/10.1007/BF02796502

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