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A major histocompatibility locus in fish: serological identification and segregation of transplantation antigens in the common carp (Cyprinus carpio L.)

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Abstract

In this study, experiments are described that were designed to investigate whether fish have an immune regulatory systems similar to the major histocompatibility complex (MHC) in higher vertebrate species. From combinations of gynogenetic carp showing either slow of fast rejection of skin transplants, the latter were chosen for alloantiserum production by hyperimmunization with peripheral blood leucocytes. The resulting alloantisera were analyzed for hemagglutinating reactivity with gynogenetic siblings and proved to be operationally monoscpecific in absorption experiments. The serologically determined carp erythrocyte specificites were shown to correspond to two codominantly expressed allelic products of a single locus and were designated K1 and K2, respectively. Flow cytometer analysis revealed that the same products are also present on leucocytes from peripheral blood, thymus, spleen, and pronephros.K1-andK2-homozygous second-generation gynogenetic siblings were used to study the histocompatibiligy nature of the K locus products. Skin transplants between K-allogeneic gynogenetic siblings were rejected significantly faster than within K-syngeneic combinations. Taken together, these data suggest that theK locus incorporates MHC class I-like characteristics.

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  1. Peter Kaastrup

    Present address: Danish Trout Culture Research Station, Brøns Møllevej 7, DK-6780, Skaerbaek, Denmark

Authors and Affiliations

  1. Department of Experimental Animal Morphology and Cell Biology, Agricultural University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands

    Peter Kaastrup, René J. M. Stet, Albert J. Tigchelaar, Egbert Egberts & Willem B. van Muiswinkel

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  1. Peter Kaastrup
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  2. René J. M. Stet
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  3. Albert J. Tigchelaar
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  5. Willem B. van Muiswinkel
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Kaastrup, P., Stet, R.J.M., Tigchelaar, A.J. et al. A major histocompatibility locus in fish: serological identification and segregation of transplantation antigens in the common carp (Cyprinus carpio L.). Immunogenetics 30, 284–290 (1989). https://doi.org/10.1007/BF02421332

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  • DOI: https://doi.org/10.1007/BF02421332

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