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Gene expression of interstitial collagenase (matrix metalloproteinase 1) in gastrointestinal tract cancers

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Abstract

Collagenolytic proteinases play an important pathological role in the invasion and metastasis of cancer cells. However, little is known about the role of interstitial collagenase (matrix metalloproteinase-1; MMP-1) in this process. To investigate the expression of the MMP-1 gene in cancer tissues, anin situ hybridization study was carried out in gastrointestinal tract cancers (one esophageal cancer, five gastric cancers, and four colorectal cancers), using a35S-labeled MMP-1 cDNA probe. The MMP-1 gene was expressed in the stromal cells of fibrous tissue around cancer nests, especially at the margin of invasion and/or within the cancer nest; however, no definite expression within cancer cells was observed. Expression of the MMP-1 gene in the stromal cells was more common in well differentiated gastric adenocarcinoma than in poorly differentiated adenocarcinomas. These findings indicate that expression of the MMP-1 gene is greater in stromal cells that are closely associated with cancer cells, suggesting a pathophysiological role of MMP-1 in the invasion and metastasis of cancer cells.

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Authors and Affiliations

  1. Department of Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan

    Yoshihide Otani & Masaki Kitajima

  2. Department of Pathology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan

    Kaori Kameyama & Yasuhiro Hosoda

  3. Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan

    Masaharu Tsuchiya

  4. Department of Community Health, School of Medicine, Tokai University, Bohseidai, 259-11, Isehara, Kanagawa, Japan

    Isao Okazaki, Masao Arai & Norihito Wada

  5. Alcohol Research Center, National Kurihama Hospital, 2769 Nobi, 239, Yokosuka, Kanagawa, Japan

    Katsuya Maruyama

  6. Department of Surgery, Tokyo Dental College, Ichikawa General Hospital, 6-7-1 Sugano, 272, Ichikawa, Chiba, Japan

    Keiichi Yoshino

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  1. Yoshihide Otani
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Otani, Y., Okazaki, I., Arai, M. et al. Gene expression of interstitial collagenase (matrix metalloproteinase 1) in gastrointestinal tract cancers. J Gastroenterol 29, 391–397 (1994). https://doi.org/10.1007/BF02361233

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  • DOI: https://doi.org/10.1007/BF02361233

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