Abstract
Hexokinase II, one member of a family of structurally similar enzymes that catalyze the phosphorylation of glucose in the 6-position, has been suggested to play a role in the pathophysiology of noninsulin-dependent diabetes mellitus (NIDDM). The gene for hexokinase II, HK2, has been previously mapped to human chromosome 2p13 by fluorescence in situ hybridization, and two-point linkage analysis has placed it near the locus for transforming growth factor α, TGFA. We now report the characterization of a (TA)n polymorphism in intron 12 ofHK2. Using multipoint analysis of CEPH family genotypes, we have determined the most likely locus order to be cen-D2S169-[D2S286-HK2]-[D2S145-D2S291]-[D2S45-D2S 101-TGFA]-tel. As HKII is a candidate gene that could contribute to the manifestation of insulin resistance and NIDDM, we genotyped 1152 Pima Indians, a Native American tribe that has the highest reported prevalence of NIDDM in the world. Although we did not detect any linkage or association ofHK2 with insulin resistance or NIDDM in the Pima Indians, the polymorphism and detailed mapping ofHK2 described in this report should prove useful in the assessment of the role of this gene in the predisposition to NIDDM in other populations.
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Ardehali, H., Tiller, G.E., Printz, R.L. et al. A novel (TA)n polymorphism in the hexokinase II gene: Application to noninsulin-dependent diabetes mellitus in the Pima Indians. Hum Genet 97, 482–485 (1996). https://doi.org/10.1007/BF02267071
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DOI: https://doi.org/10.1007/BF02267071