Summary
Eight bovine ovaries with a corpus luteum were perfused for 4 h in a haemoglobin-free semi-synthetic perfusion medium in a closed circuit. After an initial prolactin(PRL)-free perfusion phase, 4.7 ng/ml ovine PRL was added in the 1st h, followed by 47 ng/ml in the 2nd h and 470 ng/ml in the 3rd h. Glucose and oxygen consumption and the production of lactate, pyruvate and CO2 were measured, while perfusion pressure and pH-value were recorded continuously. Under the influence of PRL anaerobic glucose metabolism was stimulated by 40.5% and oxidative phosphorylation was inhibited. Energy production from aerobic glucose metabolism rose by only 0.25%. Unlike PRL, Human Menopausal Gonadotropin (hMG) and Human Chorionic Gonadotropin (hCG) stimulated aerobic metabolism. This may indicate that PRL is the “older” hormone in phylleogenetic terms.
Similar content being viewed by others
References
Armstrong DT, Knudsen KA, Miller LS (1969) Regulation of lipid metabolism and progesterone production of rat corpora lutea and ovarian interstitial elements by prolactin and luteinizing hormone. Endocrinology 85: 393–401
Armstrong DT, Knudsen KA, Miller LS (1970) Effects of prolactin upon cholesterol metabolism and progesterone biosynthesis in corpora lutea of rats hypophysectomised during pseudopregnancy. Endocrinology 86: 634–641
Behrman HR, Orczyk GP, Macdonald GJ, Greep RO (1970) Prolactin induction of enzyme controlling luteal cholesterol ester turnover. Endocrinology 87: 1251–1256
Jacobs HS (1975) Female hypogonadism. J Endocrinol 66: 12–13
L'Hermite M, Caufriez A, Robyn C (1977) Pathophysiology of human prolactin secretion with special reference to prolactin-secreting pituitary adenomas and isolated galactorrhoea. In: Crosignani PG, Robyn C (eds) Prolactin and human reproduction. Academic Press, London New York, p 179
McNatty KP, Sawers RS (1975) Relationship between the endocrine environment within the graafian follicle and the subsequent rate of progesterone secretion by human granulosa cells in vitro. J Endocrinol 66: 391–400
McNatty KP, Sawers RS, McNeilly AS (1974) A possible role for prolactin in the control of steroid secretion by human graafian follicle. Nature 250: 653–655
McNatty KP, Neal P, Baker TG (1976) Effect of prolactin on the production of progesterone by mouse ovaries in vitro. J Reprod Fertil 47: 155–156
McNatty KP, Sawers RS, McNeilly AS (1977) Prolactin and progesterone secretion by human granulosa cells in vitro. In: Crosignani PG, Robyn C (eds) Prolactin and human reproduction. Academic Press, London New York, pp 109–117
Saito T, Saxena BB (1975) Specific receptors for prolactin in the ovary. Acta Endocrinol (Copenh) 80: 126–137
Stähler E, Huch A (1971) Untersuchungen an isoliert perfundierten Ovarien des Rindes. Arch Gynäkol 211: 527
Stähler E, Buchholz R, Huch A (1971) Untersuchungen über den Energiestoffwechsel in isoliert perfundierten Ovarien des Menschen. Arch Gynäkol 211: 576
Stähler E, Spätling L, Bethge HD, Daume E, Buchholz R (1974) Induction of ovulation in human ovaries perfused in vitro. Arch Gynäkol 217: 1–15
Thorner MO, Besser GM, Jones AE, Dacie J (1975) Bromocriptine treatment of female infertility: Report of 13 pregnancies. Br Med J 4: 694–697
Author information
Authors and Affiliations
Additional information
Supported by the Deutsche Forschungsgemeinschaft
Rights and permissions
About this article
Cite this article
Spätling, L., Stähler, E., Vilmar, W. et al. Influence of prolactin on metabolism and energy production in perfused corpus luteum bearing bovine ovaries. Arch. Gynecol. 231, 263–267 (1982). https://doi.org/10.1007/BF02111724
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02111724