Abstract
The isolated bowel segment (IBS) is an amesenteric segment of bowel that is devoid of extrinsic nerves and yet is viable with motility and absorption preserved after its mesentery is completely severed. The IBS is created by initial coaptation of a loop of bowel to a host organ, such as muscle (Iowa model I), liver (Iowa model II), or intestine, and secondary severance of its mesentery several weeks later. In previous studies using Iowa models I and II, the viability, motility, and absorption of the IBS were preserved by vascular collaterals, which form across the coaptation. In rats, an IBS was created in the jejunum by initial enteroenteropexy (Iowa model III), followed by its mesenteric division five weeks later. At the second laparotomy, bipolar electrodes were implanted in the IBS and the adjacent jejunum. One week later, myoelectrical recording was performed during a fasting state. Coordinated aborad propagation of migrating motor complex (MMC) was observed in the IBS. The MMC period was 16.9 ± 1.7 and 19.3 ± 0.8 min in the intact bowel, and 22.8 ± 0.8 min in the IBS (P=0.1). After feeding, the MMC in the IBS was replaced by irregular spike burst activity similar to that observed in the intact bowel. This study concludes that the IBS Iowa model III can be used for studies of bowel physiology.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kimura K, Soper RT: Isolated bowel segment (model I): Creation by myoenteropexy. J Pediatr Surg 25:1512–1513, 1990
Ienaga T, Kimura K, Hashimoto K, Lee SC, Brakstad M, Soper RT: Isolated bowel segment (Iowa model I): Technique and histologic studies. J Pediatr Surg 25:902–904, 1990
Yamazato M, Kimura K, Yoshino H, Soper RT: The isolated bowel segment (Iowa model II) created in the functioning bowel. J Pediatr Surg 26:780–783, 1991
Yoshino H, Kimura K, Yamazato M, Scott D, Soper RT: The isolated bowel segment (Iowa model II): Absorption studies for glucose and leucine. J Pediatr Surg 26:1372–1375, 1991
Yamazato M, Kimura K, Yoshino H, Murr M, Ellsbury D, Soper RT: The isolated bowel segment (Iowa model II): Motility across the anastomosis with or without mesenteric division. J Pediatr Surg 27:691–695, 1992
Kimura K, Soper RT: A new bowel elongation technique for the short bowel syndrome using the isolated bowel segment Iowa models. J Pediatr Surg 28:792–794, 1993
Kottmier PK, Breenon WS, Nakajima N, et al: Function of replanted ileal segments in the dog. J Pediatr Surg 6:345–353, 1971
Cohen BE, Barrera A: Survival of transferred intestinal segments after vascular pedicle interruption. Plast Reconstr Surg 79:610–615, 1987
Sarr MG, Kelly KA: Myoelectric activity of the autotrans-planted canine jejunoileum. Gastroenterology 81:303–310, 1981
Murr M, Kimura K, Ellsbury D, Yoshino H, Yamazato M, Soper RT: Absorption in the isolated bowel segment. J Pediatr Gastroenterol and Nutr 17:182–185, 1993
Diamant SC, Scott RB: Migrating action potentials complexes—a feature of normal jejunal myoelectrical activity in the rat. Can J Pharmacol 65:2269–2273, 1985
Ruckebusch M, Fioramonti J: Electric spiking activity and propulsion in small intestine in fed and fasted rats. Gastroenterology 68:1500–1508, 1975
Sarna SK, Daniel EE, Kingma YJ: Simulation of slow wave electrical activity of small intestine. Am J Physiol 221:166–173, 1971
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
El-Murr, M., Kimura, K., Ellsberg, D. et al. Motility of isolated bowel segment Iowa model III. Digest Dis Sci 39, 2619–2623 (1994). https://doi.org/10.1007/BF02087699
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02087699