Summary
A proteolysed form of fructose 1,6-bisphosphatase (Fru-P2ase) has been detected and characterized in human term placenta. The extract was found to contain very low levels of activity with an alkaline pH optimum. Western blotting demonstrated a polypeptide of Mr 26,000, instead of the subunit of Mr 36,000 observed in native mammalian Fru-P2ases.
Similar content being viewed by others
Literatur
Horecker, B. L., Melloni, E., and Pontremoli, S., Adv. Enzymol.42 (1975) 193.
Tejwani, G. A., Adv. Enzymol.54 (1983) 121.
DiPietro, D. L., Gutierrez-Correa, J., and Thaidigsman, J. H., Biochem. J.103 (1967) 246.
Walker, D. G., Lea, M. A., Rossiter, G., and Addison, M. E. B., Archs Biochem. Biophys.120 (1967) 646.
Bagewadikar, R. S., Sharma, C., and Nadkarni, G. B., Indian J. exp. Biol.15 (1977) 27.
Bagewadikar, R. S., Sharma, C., and Nadkarni, G. B., Indian J. exp. Biol.15 (1977) 55.
Pontremoli, S., Traniello, S., Luppis, B., and Wood, W. A., J. biol. Chem.240 (1965) 3459.
Annamalai, A., Tsolas, O., and Horecker, B. L., Archs Biochem. Biophys.183 (1977) 48.
Bradford, M. M., Analyt. Biochem.72 (1976) 248.
Laemmli, U. K., Nature227 (1970) 680.
Towbin, H., Staehelin, T., and Gordon, J., Proc. natl. Acad. Sci. USA76 (1979) 4350.
Black, W. J., Van Tol, A., Fernando, J., and Horecker, B. L., Archs Biochem. Biophys.151 (1972) 576.
Engvall, E., and Perlmann, P., J. Immunol.109 (1972) 129.
Dzugaj, A., and Kochman, M., Biochim. biophys. Acta614 (1980) 407.
Lazo, P. S., Tsolas, O., Sun, S. C., Pontremoli, S., and Horecker, B. L., Archs Biochem. Biophys.188 (1978) 308.
Crivellaro, O., Lazo, P. S., Tsolas, O., Pontremoli, S., and Horecker, B. L., Archs Biochem. Biophys.189 (1978) 490.
Nakashima, K., and Ogino, K., J. Biochem. (Tokyo)75 (1974) 355.
Traniello, S., Pontremoli, S., Tashima, Y., and Horecker, B. L., Archs Biochem. Biophys.146 (1971) 161.
Byrne, W. L., Rajagopalan, G. T., Griffin, L. D., Ellis, E. H., Harris, T. M., Hochachka, P., Reid, L., and Geller, A. M., Archs Biochem. Biophys.146 (1971) 118.
Pontremoli, S., Melloni, E., Salamino, F., Sparatore, B., Michetti, M., and Horecker, B. L., Archs Biochem. Biophys.214 (1982) 376.
Taketa, K., and Pogell, B. M., Biochem. biophys. Res. Commun.12 (1963) 229.
Krebs, H. A., Proc. Roy. Soc. (London) Series B,159 (1964) 545.
Van Shaftingen, E., and Hers, H. G., Proc. natl Acad. Sci. USA.78 (1981) 2861.
Pilkis, S. J., El-Maghrabi, M. R., Pilkis, J., and Claus, T., J. biol. Chem.256 (1981) 3619.
Ghosh, N. K., and Fishman, W. H., J. biol. Chem.241 (1966) 2516.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Papamarcaki, T., Tsolas, O. Demonstration of fructose 1,6-bisphosphatase in human term placenta. Experientia 47, 67–68 (1991). https://doi.org/10.1007/BF02041255
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02041255