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Chromosomal mapping of T-DNA inserts in transgenicPetunia byin situ hybridization

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Abstract

The chromosomal location of T-DNa inserts in ten independently derived and confirmed transgenic plants ofP. hybrida was detected byin situ hybridization. Nine transgenic plants had the T-DNA inerts at single sites distributed among each of the seven chromosomes; in one plant the T-DNA inserts were detected on two different chromosomes. Although the T-DNA inserts were integrated randomly among the chromosomes, seven of the 11 total inserts were located at or near the telomere. Thus, T-DNA inserts appear to have potential for tagging chromosomes and chromosome fragments.

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Wang, J., Lewis, M.E., Whallon, J.H. et al. Chromosomal mapping of T-DNA inserts in transgenicPetunia byin situ hybridization. Transgenic Research 4, 241–246 (1995). https://doi.org/10.1007/BF01969117

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  • DOI: https://doi.org/10.1007/BF01969117

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